Open Access

Comparison between surface and bead-based MALDI profiling technologies using a single bioinformatics algorithm

  • Christelle Reynès1,
  • Stéphane Roche2, 3,
  • Laurent Tiers2,
  • Robert Sabatier1,
  • Patrick Jouin4,
  • Nicolas Molinari5, 6, 7 and
  • Sylvain Lehmann2, 3, 6Email author
Clinical Proteomics2:BF02752497

DOI: 10.1007/BF02752497


In this manuscript, we compared serum profiles obtained with two related technologies, SELDI-TOF and Clinprot, using a single bioinformatic algorithm. These two approaches rely on mass spectrometry to detect proteins and peptides initially selected by binding to various chromatographic matrices. They are proposed by two different companies, and they are competing for being the reference in high throughput serum profiling for clinical proteomics. This independent evaluation of these two technologies put the light on some of their differences, suggests that they address different proteome fractions and, thus, could be complementary. Taken together, our data could contribute to the parameters relevant for the choice of one technology or the other.