Fig. 1
High-abundance protein depletion of lung fluid samples. Two-dimensional electrophoresis of undepleted ARDS lung fluid samples is limited by the presence of high-abundance serum proteins. Depletion of these proteins using an immunoaffinity column resulted in a threefold improvement in detection of low-abundance proteins. The high-abundance protein fraction was analyzed for the presence of low-abundance proteins. Only four protein spots representing three low-abundance proteins were found in the high-abundance fraction