Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

Ramachandran, Prasanna; Boontheung, Pinmanee; Pang, Eric; Yan, Weihong; Wong, David T.; Loo, Joseph A.

doi:10.1007/s12014-008-9005-0

Clinical Proteomics

Table 3 N-linked glycoproteins identified in submandibular salivary fluid

From: Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

IPI accession number	Protein name	MW (Da)	Formerly N-glycosylated peptide sequence^a	Formerly N-glycosylated peptide score^b	Nonformerly glycosylated peptides identified
IPI00003351	Extracellular matrix protein 1	60,635	HIPGLIHN*MTAR	62	0
IPI00004573	Polymeric-immunoglobulin receptor	83,262	ANLTNFPENGTFVVNIAQLSQDDSGR	105	2
			IIEGEPNLKVPGN*VTAVLGETLK	108
			LSLLEEPGN*GTFTVILNQLTSR	97
			QIGLYPVLVIDSSGYVNPN*YTGR	127
			VPGN*VTAVLGETLK	116
			VPGN*VTAVLGETLKVPCHFPCK	52
			WN*NTGCQALPSQDEGPSK	45
IPI00010697	Isoform alpha-6X1X2B of integrin alpha-6	126,539	EINSLN*LTESHNSR	31	0
IPI00011229	Cathepsin D	49,768	GSLSYLN*VTR	68	0
IPI00012887	Cathepsin L	37,540	YSVAN*DTGFVDIPKQEK	45	0
IPI00017601	Ceruloplasmin	122,128	EHEGAIYPDN*TTDFQR	63	0
IPI00022431	Alpha-2-HS-glycoprotein	39,300	AALAAFNAQNN*GSNFQLEEISR	41	0
IPI00022488	Hemopexin	51,643	ALPQPQN*VTSLLGCTH	27	0
IPI00023673	Galectin-3 binding protein	65,289	AAIPSALDTN*SSK	58	0
			ALGFEN*ATQALGR	84
			GLN*LTEDTYKPR	60
			EPGSN*VTMSVDAECVPMVR	44
IPI00025023	Lactoperoxidase	80,237	KPALGAAN*R	46	0
			KPSPCEFIN*TTAR	51
			ASLTN*VTDPSLDLTSLSLEVGCGAPAPVVR	96
			IVGYLNEEGVLDQN*R	100
			LRN*LSSPLGLMAVNQEVSDHGLPYLPYDSK	58
IPI00031121	Carboxypeptidase E	53,117	DLQGNPIAN*ATISVEGIDHDVTSAK	49	0
IPI00031121	Carboxypeptidase E	53,117	GN*ETIVNLIHSTR	44	0
IPI00032292	Metalloproteinase inhibitor 1	23,156	FVGTPEVN*QTTLYQR	96	0
IPI00060143	Protein FAM3D	24,947	GLNIALVN*GTTGAVLGQK	73	0
IPI00166729	Alpha-2-glycoprotein 1, zinc	34,223	FGCEIENN*R	48	3
IPI00166729	Alpha-2-glycoprotein 1, zinc	34,223	DIVEYYNDSNGSHVLQGR	66	3
IPI00168728	FLJ00385 protein (fragment)	56,111	EEQFN*STFR	51	0
IPI00171411	Golgi membrane protein 1	42,917	AVLVNN*ITTGER	70	0
IPI00178926	Immunoglobulin J chain	18,087	EN*ISDPTSPLR	59	0
IPI00178926	Immunoglobulin J chain	18,087	IIVPLNNREN*ISDPTSPLR	77	0
IPI00218413	Biotinidase	61,093	NPVGLIGAEN*ATGETDPSHSK	58	0
IPI00242956	Fc fragment of IgG binding protein	571,718	LLISSLSESPASVSILSQADN*TSK	92	0
			VITVQVAN*FTLR	62
			VTVRPGESVMVN*ISAK	30
			VVTVAALGTN*ISIHKDEIGK	67
IPI00291410	Isoform 1 of long palate, lung, and nasal epithelium carcinoma-associated protein 1	52,408	DHN*ATSILQQLPLLSAMR	31	0
IPI00296099	Thrombospondin-1	129,300	VVN*STTGPGEHLR	70	0
IPI00295105	Carbonic anhydrase VI	35,343	GLN*MTGYETQAGEFPMVNNGHTVQIGLPSTMR	43	0
IPI00296654	Bactericidal/permeability-increasing protein-like 1	49,100	LLAAAN*FTFK	74	0
			LGATPVAMLHTNN*ATLR	68
			SDDNLLN*TSALGR	56
IPI00297910	Tumor-associated calcium signal transducer 2	35,687	HRPTAGAFN*HSDLDAELR	43	0
IPI00299729	Transcobalamin I	48,164	ADEGSLKN*ISIYTK	64	0
IPI00299729	Transcobalamin I	48,164	MN*DTIFGFTMEER	92	0
IPI00298828	Beta-2-glycoprotein 1	38,273	VYKPSAGN*NS		0
IPI00300786	Salivary alpha-amylase	57,731	NVVDGQPFTNWYDN*GSNQVAFGR	79	6
IPI00304557	Short palate, lung, and nasal epithelium carcinoma associated protein 2	26,995	GLNLSFPVTAN*VTVAGPIIGQIINLK	84	2
IPI00328460	Transmembrane protease, serine 11B	46,235	MLNAFQNSSIYK	64	0
IPI00328460	Transmembrane protease, serine 11B	46,235	LLPNAN*GSNVQLQLK	80	0
IPI00328960	Similar to LOC147645 protein	91,568	FVTAGSN*VTLR	69	0
IPI00333140	Delta-notch-like EGF repeat-containing transmembrane	78,438	LVSFEVPQN*TSVK	48	0
IPI00335356	IGHM protein	64,998	GLTFQQN*ASSMCVPDQDTAIR	60	0
IPI00374315	Hypothetical LOC389429	37,902	IILN*QTAR	63	0
IPI00384872	UDP-glucuronosyltransferase 1–7	59,779	YTGTRPSNLAN*NTILVK	46	0
IPI00400826	Clusterin	57,796	LAN*LTQGEDQYYLR	81	0
IPI00418512	DMBT1	193,867	LVNLN*SSYGLCAGR	101
IPI00418512	DMBT1	193,867	QADN*DTIDYSNFLTAAVSGGIIK	93
IPI00419215	Alpha-2-macroglobulin like protein 1	161,105	LGHIN*FTISTK	49	0
IPI00419215	Alpha-2-macroglobulin like protein 1	161,105	QGN*GTFVQTDKPLYTPGQQVYFR	72	0
IPI00423461	Hypothetical protein DKFZp686C02220	54,126	HYTN*SSQDVTVPCR	55	0
IPI00431645	HP protein	45,177	NLFLN*HSENATAK	39	0
IPI00431645	HP protein	45,177	VVLHPN*YSQVDIGLIK	57	0
IPI00550991	Alpha-1-antichymotrypsin	50,566	YTGN*ASALFILPDQDK	36	0
IPI00553177	Alpha-1-antitrypsin	46,737	YLGN*ATAIFFLPDEGK	108	0
IPI00553177	Alpha-1-antitrypsin	46,737	ADTHDEILEGLNFN*LTEIPEAQIHEGFQELLR	67	0
IPI00641229	Ig alpha-2 chain C region	53,868	LAGKPTHVN*VSVVMAEVDGTCY	81	0
			LSLHRPALEDLLLGSEAN*LTCTLTGLR	110
			TPLTAN*ITK	57
IPI00643034	Splice isoform 1 of phospholipid transfer protein	54,705	VSN*VSCQASVSR	90	0
IPI00643034	Splice isoform 1 of phospholipid transfer protein	54,705	IYSN*HSALESLALIPLQAPLK	64	0
IPI00855918	Mucin 5, subtype B, tracheobronchial	590,122	AFGQFFSPGEVIYN*K	80	1
			AQGLVLEASN*GSVLINGQR	141
			DIECQAESFPN*WTLAQVGQK	86
			GN*CTYVLMR	65
			LDGPTEQCPDPLPLPAGN*CTDEEGICHR	24
			LPYSLFHN*NTEGQCGTCTNNQR	20
			QVNETWTLENCTVAR	68
			VVLLDPKPVAN*VTCVNK	81

^aN* indicates the site of former N-linked glycosylation
^bMascot score listed in parentheses

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ISSN: 1559-0275

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