Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

Ramachandran, Prasanna; Boontheung, Pinmanee; Pang, Eric; Yan, Weihong; Wong, David T.; Loo, Joseph A.

doi:10.1007/s12014-008-9005-0

Clinical Proteomics

Table 4 N-linked glycoproteins identified in sublingual salivary fluid

From: Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

IPI accession number	Protein name	MW (Da)	Formerly N-glycosylated peptide sequence^a	Formerly N-glycosylated peptide score^b	Nonformerly glycosylated peptides identified
IPI00000877	Hypoxia-upregulated protein	111,266	VFGSQN*LTTVK	79	0
IPI00000877	Hypoxia-upregulated protein	111,266	LSALDNLLN*HSSMFLK	63	0
IPI00002818	Isoform 1 of kallikrein 11	27,448	CAN*ITIIEHQK	36	0
IPI00003351	Extracellular matrix protein 1	60,635	HIPGLIHN*MTAR	65	0
IPI00003351	Extracellular matrix protein 1	60,635	HKHIPGLIHN*MTAR	37	0
IPI00004573	Polymeric-immunoglobulin receptor	83,262	ANLTNFPENGTFVVNIAQLSQDDSGR	106	1
			GLSFDVSLEVSQGPGLLN*DTK	49
			IIEGEPNLKVPGN*VTAVLGETLK	115
			LSLLEEPGN*GTFTVILNQLTSR	114
			QIGLYPVLVIDSSGYVNPN*YTGR	122
			VPGN*VTAVLGETLK	105
			VPGN*VTAVLGETLKVPCHFPCK	82
			WN*NTGCQALPSQDEGPSK	54
IPI00010697	Isoform alpha-6X1X2B of integrin alpha-6	41,000	EINSLNLTESHNSR	32	0
IPI00011229	Cathepsin D	44,524	GSLSYLN*VTR	68	0
IPI00021891	Splice isoform 1 of fibrinogen gamma chain	51,549	DLQSLEDILHQVEN*K	46	0
IPI00021891	Splice isoform 1 of fibrinogen gamma chain	51,549	VDKDLQSLEDILHQVEN*K	58	0
IPI00022229	Apolipoprotein B-100	515,241	FVEGSHN*STVSLTTK	29	0
IPI00022431	Alpha-2-HS-glycoprotein	39,300	AALAAFNAQNN*GSNFQLEEISR	104	0
IPI00022463	Serotransferrin	77,000	QQQHLFGSN*VTDCSGNFCLFR	82	0
IPI00022463	Serotransferrin	77,000	CGLVPVLAENYN*K	43	0
IPI00022488	Hemopexin	51,643	ALPQPQN*VTSLLGCTH	63	0
IPI00022974	Prolactin-inducible protein	16,562	TFYWDFYTN*R	43	0
IPI00023673	Galectin-3 binding protein	65,289	AAIPSALDTN*SSK	58	0
			ALGFEN*ATQALGR	94
			GLN*LTEDTYKPR	39
			YKGLN*LTEDTYKPR	36
IPI00025023	Lactoperoxidase	80,237	ASLTN*VTDPSLDLTSLSLEVGCGAPAPVVR	93	1
			IVGYLNEEGVLDQN*R	110
			KPSPCEFIN*TTAR	74
			LRN*LSSPLGLMAVNQEVSDHGLPYLPYDSK	57
IPI00025846	Splice isoform 1 of desmocollin-2	99,899	NGIYN*ITVLASDQGGR	65	0
IPI00025846	Splice isoform 1 of desmocollin-2	99,899	AN*YTILK	32	0
IPI00027192	Procollagen-lysine	83,497	EQIN*ITLDHR	38	0
IPI00030847	Transmembrane 9 superfamily protein member 3	67,843	IVDVN*LTSEGK	60	0
IPI00031121	Carboxypeptidase E	53,117	GN*ETIVNLIHSTR	44	0
IPI00031547	Desmoglein 3	107,435	LPAVWSITTLN*ATSALLR	80	0
IPI00032258	Complement C4	192,650	GLN*VTLSSTGR	55	0
IPI00032292	Metalloproteinase inhibitor 1	23,156	FVGTPEVN*QTTLYQR	87	0
IPI00060143	Protein FAM3D	24,947	GLNIALVN*GTTGAVLGQK	116	0
IPI00166729	Alpha-2-glycoprotein 1, zinc	34,223	FGCEIENN*R	50	0
IPI00168728	FLJ00385 protein (fragment)	56,111	EEQFN*STFR	51	0
IPI00171411	Golgi membrane protein 1	49,768	AVLVNN*ITTGER	89	0
IPI00171411	Golgi membrane protein 1	49,768	LQQDVLQFQKN*QTNLER	43	0
IPI00178926	Immunoglobulin J chain	18,087	IIVPLNNREN*ISDPTSPLR	62	0
IPI00178926	Immunoglobulin J chain	18,087	EN*ISDPTSPLR	55	0
IPI00242956	IgGFc binding protein	571,718	LLISSLSESPASVSILSQADN*TSKK	40	0
IPI00242956	IgGFc binding protein	571,718	VTVRPGESVMVN*ISAK	47	0
IPI00291410	Isoform 1 of long palate, lung, and nasal epithelium carcinoma-associated protein 1	54,878	DHN*ATSILQQLPLLSAMR	46	0
IPI00295105	Carbonic anhydrase VI	35,343	GLN*MTGYETQAGEFPMVNNGHTVQIGLPSTMR	39	0
IPI00296099	Thrombospondin 1	129,330	VVNSTTGPGEHLR	73	0
IPI00296654	Bactericidal/permeability-increasing protein-like 1	49,100	LLAAAN*FTFK	72	0
IPI00296654	Bactericidal/permeability-increasing protein-like 1	49,100	LGATPVAMLHTNN*ATLR	66	0
IPI00297910	Tumor-associated calcium signal transducer 2	35,687	HRPTAGAFN*HSDLDAELR	65	0
IPI00298082	Calcium-activated chloride channel protein 2	101,288	AAN*SSVPPITVNAK	44	0
			HSN*GSYSAFGER	51
			DSFDDALQVN*TTDLSPK	67
IPI00298828	Beta-2-glycoprotein I	38,273	VYKPSAGN*NSLYR	79	0
IPI00299729	Transcobalamin I	48,164	ADEGSLKN*ISIYTK	73	0
IPI00299729	Transcobalamin I	48,164	MN*DTIFGFTMEER	96	0
IPI00300786	Salivary alpha-amylase	57,731	NVVDGQPFTNWYDN*GSNQVAFGR	93	1
IPI00303333	Platelet receptor GI24	33,901	HGLESASDHHGN*FSITMR	41	0
IPI00304557	Short palate, lung, and nasal epithelium carcinoma associated protein 2	26,995	GLNLSFPVTANVTVAGPIIGQIINLK	82	2
IPI00328460	Transmembrane protease, serine 11B	46,235	LLPNAN*GSNVQLQLK	83	0
IPI00328460	Transmembrane protease, serine 11B	46,235	MLNAFQN*SSIYK	59	0
IPI00328960	Predicted: hypothetical protein XP_085831	91,568	FVTAGSN*VTLR	50	0
IPI00332845	Leucine-rich repeats and calponin homology (CH) domain containing 2	69,610	KLPPGLLAN*FTLLR	77	0
IPI00335356	IGHM protein	49,526	GLTFQQN*ASSMCVPDQDTAIR	47	0
IPI00374315	Uncharacterized protein C6orf58	37,902	IILN*QTAR	62	0
IPI00384872	UDP-glucosyltransferase 1–7	59,779	YTGTRPSNLAN*NTILVK	33	0
IPI00400826	Clusterin	57,796	LAN*LTQGEDQYYLR	58	0
IPI00400826	Clusterin	57,796	MLN*TSSLLEQLNEQFNWVSR	99	0
IPI00418512	DMBT1	193,867	LVNLN*SSYGLCAGR	99	0
IPI00418512	DMBT1	193,867	QADN*DTIDYSNFLTAAVSGGIIK	92	0
IPI00419215	Alpha-2-macroglobulin like protein 1	149,965	QGN*GTFVQTDKPLYTPGQQVYFR	72	0
			LGHIN*FTISTK	39
			HSN*GSYSAFGER	51
IPI00431645	HP protein	31,362	VVLHPN*YSQVDIGLIK	53	0
IPI00431645	HP protein	31,362	NLFLNHSENATAK	41	0
IPI00550991	Alpha-1-antichymotrypsin	50,596	FN*LTETSEAEIHQSFQHLLR	58	0
IPI00641229	Ig alpha-2 chain C region	53,868	LAGKPTHVN*VSVVMAEVDGTCY	97	3
IPI00641229	Ig alpha-2 chain C region	53,868	LSLHRPALEDLLLGSEAN*LTCTLTGLR	104	3
IPI00553177	Alpha-1-antitrypsin	46,737	ADTHDEILEGLNFN*LTEIPEAQIHEGFQELLR	51	0
IPI00553177	Alpha-1-antitrypsin	46,737	YLGN*ATAIFFLPDEGK	101	0
IPI00643034	Splice isoform 1 of phospholipid transfer protein	54,705	GKEGHFYYN*ISEVK	40	0
			GAFFPLTERN*WSLPNR	41
			IYSN*HSALESLALIPLQAPLK	47
			VSN*VSCQASVSR	110
IPI00855918	Mucin 5, subtype B, tracheobronchial	590,122	AFGQFFSPGEVIYN*K	75	0
			AFGQFFSPGEVIYN*KTDR	82
			AQGLVLEASN*GSVLINGQR	137
			DIECQAESFPN*WTLAQVGQK	84
			FGNLSLYLDNHYCTASATAAAAR	99
			GN*CTYVLMR	46
			LDGPTEQCPDPLPLPAGN*CTDEEGICHR	28
			LPYSLFHN*NTEGQCGTCTNNQR	31
			QVNETWTLEN*CTVAR	70
			VVLLDPKPVAN*VTCVNK	77

^aN* indicates the site of former N-linked glycosylation
^bMascot score listed in parentheses

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ISSN: 1559-0275

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