Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

Ramachandran, Prasanna; Boontheung, Pinmanee; Pang, Eric; Yan, Weihong; Wong, David T.; Loo, Joseph A.

doi:10.1007/s12014-008-9005-0

Clinical Proteomics

Table 5 Combined list of all N-Glycoproteins identified in human salivary fluids

From: Comparison of N-linked Glycoproteins in Human Whole Saliva, Parotid, Submandibular, and Sublingual Glandular Secretions Identified using Hydrazide Chemistry and Mass Spectrometry

IPI accession number	Protein name	MW (Da)	Formerly N-glycosylated peptide^a	Formerly N-glycosylated peptide sequence	Formerly N-glycosylated peptides detected^b
IPI accession number	Protein name	MW (Da)	Formerly N-glycosylated peptide^a	Formerly N-glycosylated peptide sequence	WS	PA	SM	SL
IPI00000877	Hypoxia-upregulated protein	111,266	VFGSQN*LTTVK	510–520	No	No	No	Yes
IPI00000877	Hypoxia-upregulated protein	111,266	LSALDNLLN*HSSMFLK	822–837	No	No	No	Yes
IPI00002818	Splice isoform 1 of kallikrein 11	27,448	CAN*ITIIEHQK	163–173	Yes	No	No	Yes
IPI00003351	Extracellular matrix protein 1	60,635	HIPGLIHN*MTAR	437–448	Yes	No	Yes	Yes
IPI00004573	Polymeric-immunoglobulin receptor	83,262	VPGN*VTAVLGETLK	466–479	Yes	Yes	Yes	Yes
			WN*NTGCQALPSQDEGPSK	498–515	Yes	Yes	Yes	Yes
			IIEGEPNLKVPGN*VTAVLGETLK	457–479	Yes	Yes	Yes	Yes
			GLSFDVSLEVSQGPGLLN*DTK	118–138	No	Yes	No	Yes
			LSLLEEPGN*GTFTVILNQLTSR	413–434	Yes	Yes	Yes	Yes
			VPGN*VTAVLGETLKVPCHFPCK	466–487	Yes	Yes	Yes	Yes
			QIGLYPVLVIDSSGYVNPN*YTGR	168–190	Yes	Yes	Yes	Yes
			ANLTNFPENGTFVVNIAQLSQDDSGR	82–107	Yes	Yes	Yes	Yes
IPI00007244	Myeloperoxidase	83,815	SCPACPGSN*ITIR	315–327	Yes	No	Yes	No
IPI00007244	Myeloperoxidase	83,815	SYN*DSVDPR	481–489	Yes	No	No	No
IPI00010697	Isoform alpha 6X1X2B of integrin alpha-6	126,539	EINSLN*LTESHNSR	925–938	No	No	Yes	No
IPI00011229	Cathepsin D	49,768	GSLSYLN*VTR	257–266	Yes	Yes	Yes	Yes
IPI00855918	Mucin 5, subtype B, tracheobronchial	590,122	VVLLDPKPVAN*VTCVNK	4,977–4,993	Yes	No	Yes	Yes
			AQGLVLEASN*GSVLINGQR	136–154	Yes	No	Yes	Yes
			GN*CTYVLMR	5,036–5,044	Yes	No	Yes	Yes
			AFGQFFSPGEVIYN*K	4,888–4,902	Yes	No	Yes	Yes
			AFGQFFSPGEVIYN*KTDR	4,888–4,905	Yes	No	Yes	Yes
			DIECQAESFPN*WTLAQVGQK	1,547–1,567	Yes	No	Yes	Yes
			QVNETWTLEN*CTVAR	4,956–4,970	Yes	No	Yes	Yes
			LDGPTEQCPDPLPLPAGN*CTDEEGICHR	237–264	Yes	No	Yes	Yes
			FGN*LSLYLDNHYCTASATAAAAR	5,054–5,076	No	No	No	Yes
			LPYSLFHN*NTEGQCGTCTNNQR	5,153–5,172	No	No	No	Yes
IPI00012887	Cathepsin L	37,540	YSVAN*DTGFVDIPK	217–230	Yes	Yes	Yes	No
IPI00013972	Carcinoembryonic antigen-related cell adhesion molecule 8		LFIPN*ITTK	284–292	Yes	No	No	No
IPI00017601	Ceruloplasmin	122,128	EN*LTAPGSDSAVFFEQGTTR	396–415	No	Yes	No	No
IPI00017601	Ceruloplasmin	122,128	EHEGAIYPDN*TTDFQR	129–144	No	Yes	Yes	No
IPI00019943	Afamin	69,024	DIENFN*STQK	28–37	Yes	No	No	No
IPI00020091	Alpha-1-acid glycoprotein 2	23,588	QNQCFYN*SSYLNVQR	87–101	Yes	No	No	No
IPI00020487	Extracellular glycoprotein lacritin	14,237	QFIEN*GSEFAQK	115–126	Yes	No	No	No
IPI00020986	Lumican	38,405	LHINHNN*LTESVGPLPK	121–137	Yes	No	No	No
IPI00021891	Splice isoform 1 of fibrinogen gamma chain	51,479	VDKDLQSLEDILHQVEN*K	62–79	Yes	Yes	No	Yes
IPI00021891	Splice isoform 1 of fibrinogen gamma chain	51,479	DLQSLEDILHQVEN*K	65–79	Yes	No	No	Yes
IPI00022229	Apolipoprotein B-100	515,241	FVEGSHN*STVSLTTK	3,405–3,419	No	No	No	Yes
IPI00022395	Complement component C9	63,133	AVN*ITSENLIDDVVSLIR	413–430	Yes	No	No	No
IPI00022417	Leucine-rich alpha-2-glycoprotein	38,944	KLPPGLLAN*FTLLR	178–191	Yes	Yes	No	Yes
IPI00022429	Alpha-1-acid glycoprotein 1	2,477	QDQCIYN*TTYLNVQR	87–101	Yes	No	No	No
IPI00022431	Alpha-2-HS-glycoprotein	39,300	AALAAFNAQNN*GSNFQLEEISR	166–187	Yes	Yes	Yes	Yes
IPI00022431	Alpha-2-HS-glycoprotein	39,300	VCQDCPLLAPLN*DTR	145–159	Yes	Yes	No	No
IPI00022463	Serotransferrin	77,000	QQQHLFGSN*VTDCSGNFCLFR	622–642	Yes	Yes	No	Yes
IPI00022463	Serotransferrin	77,000	CGLVPVLAENYN*K	421–433	Yes	Yes	No	Yes
IPI00022488	Hemopexin	51,643	ALPQPQN*VTSLLGCTH	747–762	Yes	Yes	Yes	Yes
IPI00022488	Hemopexin	51,643	SWPAVGN*CSSALR	181–193	Yes	No	No	No
IPI00643034	Splice isoform 1 of phospholipid transfer protein	54,705	VSN*VSCQASVSR	141–152	No	Yes	Yes	Yes
			GAFFPLTERN*WSLPNR	236–244	No	Yes	No	Yes
			IYSN*HSALESLALIPLQAPLK	395–415	No	Yes	Yes	Yes
			GKEGHFYYN*ISEVK	56–69	No	Yes	No	Yes
IPI00022974	Prolactin-inducible protein	16,562	TFYWDFYTN*R	97–106	Yes	No	No	Yes
IPI00023673	Galectin-3 binding protein	65,289	ALGFEN*ATQALGR	64–76	Yes	Yes	Yes	Yes
			EPGSN*VTMSVDAECVPMVR	188–206	Yes	No	Yes	No
			YKGLN*LTEDTYKPR	394–405	Yes	Yes	No	Yes
			GLN*LTEDTYKPR	396–407	Yes	Yes	Yes	Yes
			AAIPSALDTN*SSK	542–554	Yes	Yes	Yes	Yes
			TVIRPFYLTN*SSGVD	571–585	Yes	No	No	No
IPI00025023	Lactoperoxidase	80,237	ASLTN*VTDPSLDLTSLSLEVGCGAPAPVVR	102–131	No	Yes	Yes	Yes
			IVGYLNEEGVLDQN*R	199–213	Yes	Yes	Yes	Yes
			LRN*LSSPLGLMAVNQEVSDHGLPYLPYDSK	320–349	Yes	Yes	Yes	Yes
			KPSPCEFIN*TTAR	350–362	Yes	Yes	Yes	Yes
IPI00025753	Desmoglein 1	113,644	TGEIN*ITSIVDR	106–117	Yes	No	No	No
IPI00025846	Splice isoform 1 of desmocollin-2	99,899	NGIYN*ITVLASDQGGR	542–557	Yes	No	No	Yes
			AN*YTILK	391–397	Yes	No	No	Yes
			AN*YTILKGNENGNFK	391–405	Yes	No	No	No
			LKAIN*DTAAR	625–634	Yes	No	No	No
IPI00027192	Procollagen-lysine	83,497	EQIN*ITLDHR	194–203	No	No	No	Yes
IPI00027486	Carcinoembryonic antigen-related cell adhesion molecule 5	76,748	TLTLFN*VTR	555–563	Yes	No	No	No
IPI00030847	Transmembrane 9 superfamily protein member 3	67,843	IVDVN*LTSEGK	170–180	No	No	No	Yes
IPI00031019	Cystatin-related epididymal spermatogenic protein	16,265	KLKPVN*ASNANVK	34–46	Yes	No	No	No
IPI00031121	Carboxypeptidase E	53,117	GN*ETIVNLIHSTR	138–150	Yes	No	Yes	Yes
IPI00031121	Carboxypeptidase E	53,117	DLQGNPIAN*ATISVEGIDHDVTSAK	382–406	Yes	No	Yes	No
IPI00031547	Desmoglein 3	107,435	NTGDIN*ITAIVDR	105–117	Yes	No	No	No
			DSTFIVN*K	453–460	Yes	No	No	No
			LPAVWSITTLN*ATSALLR	535–552	Yes	No	No	Yes
IPI00032258	Complement C4	192,650	GLN*VTLSSTGR	1,326–1,336	Yes	Yes	No	Yes
IPI00032292	Metalloproteinase inhibitor 1	23,156	FVGTPEVN*QTTLYQR	46–60	Yes	Yes	Yes	Yes
IPI00032328	Splice Isoform 1 of kininogen	71,900	LNAENN*ATFYFK	289–300	No	Yes	No	No
IPI00060143	Protein FAM3D	24,947	GLNIALVN*GTTGAVLGQK	100–117	Yes	No	Yes	Yes
IPI00166729	Alpha-2-glycoprotein 1, zinc	34,223	DIVEYYNDSN*GSHVLQGR	100–117	Yes	No	Yes	No
			FGCEIENN*R	118–126	Yes	No	Yes	Yes
			FGCEIENN*RSSGAFWK	118–133	Yes	No	No	No
IPI00171411	Golgi membrane protein 1	49,768	AVLVNN*ITTGER	113–124	Yes	No	Yes	Yes
IPI00171411	Golgi membrane protein 1	49,768	LQQDVLQFQKN*QTNLER	143–152	Yes	No	No	Yes
IPI00178926	Immunoglobulin J chain	18,087	EN*ISDPTSPLR	70–80	Yes	Yes	Yes	Yes
IPI00178926	Immunoglobulin J chain	18,087	IIVPLNNREN*ISDPTSPLR	62–80	Yes	Yes	Yes	Yes
IPI00218413	Biotinidase	61,093	NPVGLIGAEN*ATGETDPSHSK	320–340	No	No	Yes	No
IPI00218460	Splice isoform 3 of attractin	158,537	IDSTGN*VTNELR	411–422	Yes	No	No	No
IPI00242956	Fc fragment of IgG binding protein	571,718	LLISSLSESPASVSILSQADN*TSK	55–78	No	No	Yes	No
			LLISSLSESPASVSILSQADN*TSKK	55–79	No	No	No	Yes
			VTVRPGESVMVN*ISAK	78–95	Yes	No	Yes	Yes
			KVTVRPGESVMVN*ISAK	79–95	Yes	No	No	No
			VVTVAALGTN*ISIHKDEIGK	1,734–1,748	Yes	No	Yes	No
			VITVQVAN*FTLR	2,511–2,522	Yes	No	Yes	No
			YLPVN*SSLLTSDCSER	5,182–5,197	Yes	No	No	No
IPI00400826	Clusterin	57,796	LAN*LTQGEDQYYLR	424–437	Yes	Yes	Yes	Yes
IPI00400826	Clusterin	57,796	MLN*TSSLLEQLNEQFNWVSR	352–364	No	Yes	No	Yes
IPI00291410	Isoform 1 of long palate, lung, and nasal epithelium carcinoma-associated protein 1	54,878	DHN*ATSILQQLPLLSAMR	46–65	Yes	Yes	Yes	Yes
IPI00291410		54,878	GDQLILNLNN*ISSDR	392–406	Yes	No	No	No
IPI00291488	Splice isoform of WAP four-disulfile core domain protein 2	12,984	TGVCPELQADQN*CTQECVSDSECADNLK	33–60	Yes	No	No	No
IPI00295105	Carbonic anhydrase VI	35,343	LENSLLDHRN*K	247–257	Yes	No	No	No
IPI00295105	Carbonic anhydrase VI	35,343	GLN*MTGYETQAGEFPMVNNGHTVQIGLPSTMR	65–96	No	Yes	Yes	Yes
IPI00296099	Thrombospondin 1	129,330	VVN*STTGPGEHLR	1,065–1,077	Yes	Yes	Yes	Yes
IPI00296654	Bactericidal/permeability-increasing protein-like 1	49,100	LGATPVAMLHTNN*ATLR	320–336	Yes	Yes	Yes	Yes
			LLAAAN*FTFK	91–100	Yes	No	Yes	Yes
			SDDNLLN*TSALGR	287–299	Yes	No	Yes	No
IPI00297910	Tumor-associated calcium signal transducer 2	35,687	HRPTAGAFN*HSDLDAELR	160–177	Yes	No	Yes	Yes
IPI00298082	Calcium activates chloride channel protein 2	101,288	AAN*SSVPPITVNAK	586–559	No	No	No	Yes
			DSFDDALQVN*TTDLSPK	802–818	Yes	No	No	Yes
			N*VSILIPENWK	75–85	No	No	No	Yes
IPI00298237	Tripeptidyl-peptidase I	61,708	FLSSSPHLPPSSYFN*ASGR	429–447	Yes	No	No	No
IPI00298497	Fibrinogen beta chain	55,892	GTAGNALMDGASQLMGEN*R	377–395	No	Yes	No	No
IPI00298828	Beta-2-glycoprotein I	38,273	VYKPSAGN*NSLYR	155–167	Yes	Yes	Yes	Yes
IPI00298828	Beta-2-glycoprotein I	38,273	LGN*WSAMPSCK	251–261	Yes	No	No	No
IPI00299547	Neutrophil gelatinase-associated lipocalin	22,774	EDKSYN*VTSVLFR	64–76	Yes	No	No	No
IPI00299547	Neutrophil gelatinase-associated lipocalin	22,774	SYN*VTSVLFR	83–92	Yes	No	No	No
IPI00299729	Transcobalamin I	48,164	ADEGSLKN*ISIYTK	209–222	Yes	Yes	Yes	Yes
			MN*DTIFGFTMEER	368–380	Yes	Yes	Yes	Yes
			N*ISIYTK	216–222	No	No	Yes	Yes
			AQKMN*DTIFGFTMEER	365–380	Yes	No	No	No
IPI00300786	Salivary alpha-amylase	57,731	NVVDGQPFTNWYDN*GSNQVAFGR	414–436	Yes	Yes	Yes	Yes
IPI00304557	Short palate, lung, and nasal epithelium carcinoma-associated protein 2	26,995	GLNLSFPVTANVTVAGPIIGQIINLK	122–147	Yes	No	Yes	Yes
IPI00304557		26,995	AEPIDDGKGLNLSFPVTANVTVAGPIIGQIINLK	114–147	Yes	No	No	No
IPI00553177	Alpha-1-antitrypsin	46,737	YLGN*ATAIFFLPDEGK	268–283	Yes	Yes	Yes	Yes
IPI00553177	Alpha-1-antitrypsin	46,737	ADTHDEILEGLNFN*LTEIPEAQIHEGFQELLR	94–125	No	Yes	Yes	Yes
IPI00303333	Platelet receptor GI24	33,901	HGLESASDHHGN*FSITMR	117–134	No	No	No	Yes
IPI00328460	Transmembrane protease, serine 11B	46,235	MLNAFQN*SSIYK	81–92	No	No	Yes	Yes
IPI00328460	Transmembrane protease, serine 11B	46,235	LLPNAN*GSNVQLQLK	102–116	No	No	Yes	Yes
IPI00328960	Similar to carcinoembryonic antigen-related cell adhesion molecule 1	91,568	FVTAGSN*VTLR	427–437	Yes	No	Yes	Yes
IPI00328960		91,568	TPASN*ISTQVSHTK	140–153	Yes	No	No	No
IPI00332845	Leucine-rich repeats and calponin homology (CH) domain containing 2	69,610	MLTYLN*ISR	158–166	No	No	No	Yes
IPI00332845		69,610	KLPPGLLAN*FTLLR	178–191	No	No	No	Yes
IPI00333140	Delta-notch-like EGF repeat-containing transmembrane	78,438	LVSFEVPQN*TSVK	215–256	Yes	Yes	Yes	No
IPI00335356	IGHM protein	49,526	GLTFQQN*ASSMCVPDQDTAIR	354–374	Yes	Yes	Yes	Yes
IPI00374315	Hypothetical LOC389429	37,902	IILN*QTAR	66–73	Yes	No	Yes	Yes
IPI00374315	Hypothetical LOC389429	37,902	MGMYKIILN*QTAR	61–73	Yes	No	No	No
IPI00384872	UDP-glucuronosyltransferase 1–7	59,779	YTGTRPSNLAN*NTILVK	334–350	No	No	Yes	Yes
IPI00641229	Ig alpha-2 chain C region	55,715	LAGKPTHVN*VSVVMAEVDGTCY	319–340	Yes	Yes	Yes	Yes
			LSLHRPALEDLLLGSEAN*LTCTLTGLR	114–140	Yes	Yes	Yes	Yes
			TPLTAN*ITK	200–208	Yes	No	Yes	No
IPI00418512	DMBT1	259,409	LVNLN*SSYGLCAGR	998–1,111	Yes	Yes	Yes	Yes
			QADN*DTIDYSNFLTAAVSGGIIK	1,298–1,320	Yes	Yes	Yes	Yes
			CSGN*ESYLWSCPHK	822–835	Yes	No	No	No
IPI00419164	Alpha-2-macroglobulin like protein 1	149,965	LGHIN*FTISTK	863–873	Yes	No	Yes	Yes
			QGN*GTFVQTDKPLYTPGQQVYFR	118–140	No	No	Yes	Yes
			HSN*GSYSAFGER	1,018–1,029	No	No	No	Yes
IPI00423461	Hypothetical protein DKFZp686C02220	54,126	HYTN*SSQDVTVPCR	249–262	No	No	Yes	No
IPI00431645	HP protein	31,362	MVSHHN*LTTGATLINEQWLLTTAK	54–77	Yes	Yes	No	No
			NLFLNHSENATAK	78–90	Yes	Yes	Yes	Yes
			VVLHPN*YSQVDIGLIK	170–186	Yes	Yes	Yes	Yes
IPI00168728	FLJ00385 protein (fragment)	56,111	EEQFN*STFR	285–294	Yes	Yes	Yes	Yes
IPI00550991	Alpha-1-antichymotrypsin	50,596	FN*LTETSEAEIHQSFQHLLR	190–219	No	Yes	No	Yes
IPI00550991	Alpha-1-antichymotrypsin	50,596	YTGN*ASALFILPDQDK	293–308	No	No	Yes	No

^aN* indicates the site of former N-linked glycosylation
^bWS Whole saliva, PA parotid, SM submandibular, SL sublingual

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