18O Labeling for a Quantitative Proteomic Analysis of Glycoproteins in Hepatocellular Carcinoma
© Humana Press 2008
Received: 18 February 2008
Accepted: 23 June 2008
Published: 18 July 2008
Quantitative proteomics using tandem mass spectrometry is an attractive approach for identification of potential cancer biomarkers. Fractionation of complex tissue samples into subproteomes prior to mass spectrometric analyses increases the likelihood of identifying cancer-specific proteins that might be present in low abundance. In this regard, glycosylated proteins are an interesting class of proteins that are already established as biomarkers for several cancers.
Materials and Methods
In this study, we carried out proteomic profiling of tumor and adjacent non-cancer liver tissues from hepatocellular carcinoma (HCC) patients. Glycoprotein enrichment from liver samples using lectin affinity chromatography and subsequent 18O/16O labeling of peptides allowed us to obtain relative abundance levels of lectin-bound proteins. As a complementary approach, we also examined the relative expression of proteins in HCC without glycoprotein enrichment. Lectin affinity enrichment was found to be advantageous to quantitate several interesting proteins, which were not detected in the whole proteome screening approach. We identified and quantitated over 200 proteins from the lectin-based approach. Interesting among these were fetuin, cysteine-rich protein 1, serpin peptidase inhibitor, leucine-rich alpha-2-glycoprotein 1, melanoma cell adhesion molecule, and heparan sulfate proteoglycan-2. Using lectin affinity followed by PNGase F digestion coupled to 18O labeling, we identified 34 glycosylation sites with consensus sequence N-X-T/S. Western blotting and immunohistochemical staining were carried out for several proteins to confirm mass spectrometry results.
This study indicates that quantitative proteomic profiling of tumor tissue versus non-cancerous tissue is a promising approach for the identification of potential biomarkers for HCC.
KeywordsHepatocellular carcinoma Lectin affinity enrichment Quantitative proteomics Mass spectrometry
The incidence of hepatocellular carcinoma is rising, making it the fifth most common malignancy worldwide . This increased incidence is attributed to viral hepatitis and carcinogenic toxins such as aflatoxin . Alpha fetoprotein (AFP), one of the tumor markers used for diagnostic purposes in hepatocellular carcinoma (HCC), is limited in its diagnostic utility because 50% (35% in Johns Hopkins Hospital ) of the patients with HCC do not show high level of AFP [4–6]. Serum level of des-gamma-carboxy prothrombin (DCP) is also shown to be useful for the diagnosis of HCC in conjunction with AFP . Several studies have reported potential diagnostic markers for HCC, which are associated with chromosomal alterations, mRNA expression alterations, protein-level alterations and post-translational modifications. Lately, proteomic strategies have been extensively used for biomarker discovery because of their ability for multiplex analysis using quantitative approaches. Quantitative proteomics using isotope-labeling techniques provide opportunities to analyze changes in protein levels in multiple samples in an unbiased fashion . However, a limitation of these techniques is that, due to the complexity of samples, least abundant proteins are not detected.
O- and N-glycosylation of proteins have been associated with cancers including HCC. For example, an overall increase in fucosylation has been reported in HCC serum proteins . Increased or altered glycosylation is also reported to be associated with secreted alpha fetoprotein  and transferrin  in HCC. Glycomic profiling is a complementary strategy used to identify cancer-specific glycans especially in ovarian cancer , HCC , and pancreatic cancer . There are several reports on the application of lectin-based enrichment method to study the glycoproteome in cancers [15, 16]. Lectin reactive form of AFP is reported to be a more sensitive marker for HCC . The profile of carbohydrate moieties of glycoproteins has also been used for differentiating normal from cancerous tissue in lung  and pancreatic cancers . Since individual lectins have their own specificity while the nature of the carbohydrates present in samples may vary dramatically, it is advantageous to use a combination of lectins for enrichment purposes. Such a multiple lectin affinity approach has been used for breast cancer biomarker analysis . A mixture of different lectins has also been used for lectin affinity enrichment method to identify biomarkers in bile secretome from cancer patients .
Stable isotope can be incorporated into proteins and peptides by different methods in vitro such as ICAT , iTRAQ , 18O-labeling methods , and in vivo labeling, using heavy-isotope-labeled amino acids in cell culture (SILAC) . Labeling of peptides in the presence of 18O-labeled water is a simple method without chemical tagging and can be readily used to analyze the proteins separated by SDS-PAGE . The present study is designed to analyze subproteome in HCC using affinity enrichment and relative quantitation based on 18O labeling. Further, analysis of glycopeptides treated with PNGase F provides definitive evidence of N-linked glycosylation . Western blotting was used to confirm the differential expression results obtained by quantitative mass spectrometry. The candidate biomarkers were screened by immunohistochemical labeling using tissue microarrays containing hepatocellular carcinoma and non-cancerous liver tissue. We validated fetuin, a glycoprotein involved in endocytosis using immunohistochemical labeling. We also identified N-glycosylated peptides with sites from lectin-enriched protein. These results demonstrate advantages of lectin affinity enrichment method before quantitative proteomics profiling for identification of potential biomarkers for cancers.
Materials and Methods
Liver tissue samples were obtained after obtaining appropriate Institutional Review Board approval. One pair of tumor tissue and patient-matched non-cancerous tissue was collected at the time of surgery from patients and snap-frozen in liquid nitrogen. Serum samples were collected from HCC patients and stored at −80°C until analysis. A total of 114 HCC samples and 79 non-cancer liver sections from two tissue microarrays were used in this study. Formalin fixed and paraffin embedded tissue microarrays (Creative Biolabs, Cat No. CBL-TMA-070 and CBL-TMA-076), which consisted of 55 tissue sections from hepatocellular carcinoma with I, II, and III stages and 20 non-cancer tissues were used for immunohistochemical labeling. Tissue microarrays obtained from Imgenex consisted of 59 tumors (13 metastatic tissues, 46 poor to well-differentiated HCC, Cat. No. IMH-318) and 59 non-cancer tissues (Cat. No. IMH-342). Frozen tissues were used for mass spectrometric analysis and validation by Western blotting.
Lectin Affinity Enrichment
Tissue lysates were prepared from fresh frozen tissues from the tumor and adjacent non-cancerous region from a patient diagnosed with HCC in the presence of cocktail protease inhibitor and 10 mM phosphate buffer pH 7.8 (wash buffer). Lysates (4 mg protein) were subsequently incubated with 100 μl of each of ConA sepharose (Amersham BioSciences), wheat germ agglutinin and jacalin agarose for 12 h at 4°C. The beads were then washed three times using wash buffer and the bound proteins eluted using a mixture of carbohydrates (100 mM each of N-acetylglucosamine, melibiose, and galactose). The eluate was dialyzed against wash buffer (three volumes) to remove free sugars, concentrated and resolved by SDS-PAGE and visualized using Colloidal Coomassie staining.
Serum samples were obtained from patients diagnosed with hepatocellular carcinoma. Pooled normal serum was obtained from Invitrogen. Serum samples were prepared by incubating 100 μl (7 mg) of serum with 0.5 ml of mixture of lectins (ConA sepharose, jacalin, wheat germ lectins) overnight at 4°C in Tris-buffered saline (0.05 M Tris–HCL, pH 7.1, 0.15 M NaCl). Beads were washed three times in Tris buffer saline and boiled in Laemmli buffer for 10 min at 95°C to elute the proteins, and eluted proteins were run on 10% SDS-PAGE for further analysis by Western blot.
18O Labeling and In-gel Trypsin Digestion
Liver tissue samples from non-cancer and HCC tissues were run in parallel lanes on an SDS-PAGE gel for comparative analysis. Two gels were used for analysis, one from whole proteome and another from lectin-enriched proteins from HCC and normal tissues. Exact size gel slices corresponding to individual protein bands from HCC and normal tissues were excised and used for in-gel trypsin digestion as explained earlier  using Stratagene prolytica 18O labeling kit. In brief, sliced colloidal Coomassie-stained gel bands were destained and completely dehydrated gel slices were incubated with trypsin in the presence of heavy or normal water overnight. After completion of digestion, peptides were extracted from each sample, dried and subjected to post-trypsin digestion. Samples were incubated with immobilized trypsin (5 μl beads per sample) in the presence of heavy or normal water for 3 h. Finally, peptides derived from adjacent bands corresponding to normal and HCC were acidified, mixed, and analyzed on a quadrupole time-of-fight (QToF) mass spectrometer.
PNGase F Treatment for Identification of N-glycosylation Sites
Lectin affinity enriched proteins from HCC tissue were separated on 1D SDS-PAGE and stained with colloidal Coomassie. Protein bands were destained, reduced, and alkylated. Gel pieces were then subjected to in-gel trypsin (Promega) digestion and extracted peptides were dried completely. For the confirmation of N-glycosylation sites, peptides were incubated with PNGase F in the presence of 18O-water at 37°C for 3 h. After incubation, peptides were analyzed by LC-MS/MS (liquid chromatography–tandem mass spectrometry).
LC-MS/MS Analysis and Quantitation
In a separate study HCC and normal whole tissue lysates were analyzed on a SDS-PAGE gel and 18O labeling was done during in-gel trypsin digestion. For the common proteins found in this experiment, we were able to compare the changes in ratios to study glycoyslation status of proteins in normal and HCC. Bands were destained using ammonium bicarbonate in 50% ACN, dehydrated by acetonitrile and dried completely. Digestion was carried out in sequence grade trypsin (Promega, Southampton, UK), 20 μg/ml in 16O-water or 18O-water. Peptides were extracted using 16O-water or 18O-water three times and dried in SpeedVac. Subsequently, postdigest-end labeling was carried using prewashed immobilized trypsin (Stratagene; 2 μl/sample). Immobilized trypsin was added to the dried peptide samples and subsequently reconstituted in 8 μl of 18O-water or 16O-water, 2 μl of acetonitrile and incubated for 4 h at room temperature. Samples were centrifuged 10,000 ×g to remove the immobilized trypsin. Supernatant was acidified with 1 μl formic acid and stored at −80°C until LC-MS/MS analysis.
For LC-MS/MS analysis, peptides from paired samples were mixed and analyzed using Micromass quadrupole time-of-flight mass spectrometer (QToF) connected to reversed-phase nano LC system (Agilent 1100). The trap column consisted of (150 μM × 3 cm, C18, 15 μm, 300A, YDAC, flow rate 1.5 μl/min) and analytical column (75 μM × 10 cm, C18, 5 μm, 300A, YDAC, flow rate 200 nl/min), solvent system included 0.4% acetic acid, 0.004% heptafluorobutyric acid). The peptides were eluted using a gradient of acetonitrile up to 45% containing 0.4% acetic acid, 0.004% heptafluorobutyric acid. LC-MS/MS analysis was based on data dependent analysis (DDA) manner, performed using a 1-s MS survey scan m/z, 300-1,800 followed by four MS/MS scans (m/z, 50–1,800) for most intense ions and precursor ions were excluded for 60 s for the next MS/MS scans. A total of 4.4 s time was allowed for MS/MS spectrum acquisition.
The identification and quantitation of the peptide was done after formatting the mass spectrometry data. LC-MS/MS data acquired using Masslynx (Micromass) were searched using Mascot (Matrixscience, Manchester, UK). While searching the database, 18O at C-terminal carboxyl group (+4 Da) and oxidation of methionine were allowed as variable modification. Relative abundance of proteins was quantitated using MSQuant software downloaded from http://msquant.sourceforge.net  and expressed as fold changes (±S.D.). Essentially, Mascot search results were parsed with LC-MS/MS instrument data file using MSQuant. The new_MSQ_quantitationModes.xml files were modified to read the mascot output files and identify the correct light and heavy isotopic MS peaks. The quantitation data was also verified by manual inspection of heavy- and light-peptide-derived MS and MS/MS spectra in MSQuant.
Validation of the mass spectrometry results was carried out for a subset of proteins by Western blotting analysis using specific antibodies. These proteins were selected based on their functional relevance and differential expression between the cancerous and normal liver tissue. Eighty micrograms of protein was resolved on SDS-PAGE and transferred electrophoretically onto a nitrocellulose membrane. The membrane was blocked using 5% bovine serum albumin prepared in phosphate-buffered saline containing 0.1% Tween 20 (PBS-T) for 1 h at room temperature. The membrane was probed with specific primary antibody for 3 h and washed thrice with PBS-T and incubated in the HRP-conjugated secondary antibody for 1 h. After the incubation, the membrane was washed thrice in PBS-T. The signal was visualized by an enhanced chemiluminescence solution and exposed to Hyperfilm. Following antibodies were used for Western blot analysis. Fetuin (AF 1184, 1:1000, R&D systems) and CSRP1 (SC-33331, 1:1000, Santa Cruz).
Immunohistochemical labeling was performed on liver tissue sections and liver cancer tissue microarrays for a subset of proteins based on their biological relevance. Tissue microarrays were obtained from Creative Biolabs, NY (tumor TMA, CBL-TMA-070 and normal TMA, CBL-TMA-076) and Imgenex, San Diego, CA (tumor TMA, IMH 318, lot CS3 and normal TMA, IMH-342 lot CSN3). The Envision kit (DAKO) was used according to the manufacturer’s specifications. Briefly, the slides were first deparaffinized by xylene and rehydrated with ethanol. Antigen retrieval was done by heating the slides in 0.01 mol/L of sodium citrate buffer for 20 min on a steamer. After blocking by peroxidase, the sections were incubated with primary antibody anti-Fetuin A (dilution 1:400). After rinsing with wash buffer, the slides were incubated with HRP-conjugated appropriate secondary antibody. The signal was developed using chromogen supplied for peroxidase. Tissue sections were observed using Nikon DS-Fi1, microscope-operated using NIS-Elements F package. The immunostaining was assessed by an experienced liver pathologist and intensity was scored as negative, weak (1+), moderate (2+), and strong (3+). The distribution of staining of cancer cells was scored as 3+ for maximum distribution and, accordingly, 2+ and 1+ score was given for lower distribution. The following antibodies were used for immunohistochemical analysis: Fetuin (1:100) and CSRP1 (1:100).
Results and Discussion
Lectin Affinity Chromatography for Enrichment of Glycoproteins
It has been established that aberrant glycosylation serves as a marker of tumor progression in cancers. Several reagents that block glycosylation have been shown to inhibit tumor metastasis. N- or O-glycosylation of membrane components play an important role in altering tumor cell adhesion or motility . In general, glycosylation modification is involved in cell adhesion, enhanced matrix destructive properties and cell motility in tumors.
A partial list of identified proteins previously reported to be overexpressed in HCC
RefSeq accession number
18O/16O ratio (tumor/non-tumor)
Heat shock protein 90 kDa alpha
Clusterin isoform 2
Complement component 4B
Superoxide dismutase 3, extracellular
ADP-ribosylation factor 5
Cofilin 1 (non-muscle)
18O Labeling and Mass Spectrometry
Partial list of proteins overexpressed in HCC
RefSeq accession number
Lectin affinity (HCC:normal)
Whole tissue analysis (HCC:normal)
Cofilin 1 (non-muscle)
H2B histone family, member A
Fibulin 1 isoform D
Transforming growth factor, beta-induced, 68 kDa
Superoxide dismutase 3, extracellular
Destrin isoform A
Centrosomal protein 110 kDa
Serpin peptidase inhibitor, clade A,
Complement component 4B
ARP1 actin-related protein 1 homolog A, centractin
Melanoma cell adhesion
Fibronectin 1 isoform 6
Clusterin isoform 2
Complement factor B
Leucine-rich alpha-2-glycoprotein 1
Alpha-2-glycoprotein 1, zinc
Coagulation factor II
Heparan sulfate proteoglycan 2
ADP-ribosylation factor 5
Heat shock 70 kDa protein 5
ADP-ribosylation factor 1
Gelsolin isoform b
Heat shock 90 kDa protein 1, beta
Laminin, alpha 4
Inter-alpha (globulin) inhibitor H4
Anti-RhD monoclonal T125 gamma1 heavy chain
Beta-galactoside-binding lectin (Galectin 1)
Elastin microfibril interfacer 1
Cysteine-rich protein 1
Overexpressed Proteins in HCC
We used the 18O-labeling method for the relative quantitation of proteins from HCC and adjacent normal tissue. Both unfractionated sample and eluates of lectin-bound proteins from HCC and adjacent normal were separated on 1D SDS-PAGE. Nearly 50% of the proteins identified from lectin affinity methods were also found in whole proteome analysis allowing the comparison of glycosylation status of these proteins in cancer. Both quantitation approaches revealed many known and novel upregulated proteins in cancer. Table 1 shows a partial list of overexpressed proteins detected in lectin affinity methods. Among them haptoglobin , alpha-2-microglobulin , tenascin C , transferrin [34, 35], fibronectin , caveolin 1 [37, 38], heat shock 70 kDa protein 5 , and ceruloplasmin  have been reported to be upregulated in HCC. We also found several interesting proteins, which are highly overexpressed and they have not been reported in HCC. These include serpin peptidase inhibitor (clade A, member 3), alpha-2-HS-glycoprotein, calumenin, cysteine and glycine-rich protein 1, beta-galactoside-binding lectin, peroxiredoxin 2, and clusterin isoform 2. Similarly, many of the proteins enriched by lectin affinity have been shown to be associated with HCC, demonstrating effectiveness of our approach. Lectin affinity method enriched several known glycosylated proteins. Galectin 1 is one such protein overexpressed (3.0-fold) in HCC, which has been reported to be overexpressed at mRNA level in HCC . Alpha-2-glycoprotein 1, zinc and alpha 1B-glycoprotein were found to be 5.0 and 3.1-fold overexpressed in HCC, respectively. Many studies on cancer-associated glycosylation reported upregulated proteins such as haptoglobin related protein and hemopexin which are known to undergo glycosylation in HCC [42, 43]. Clusterin isoform 2 (apolipoprotein J), a sulfated glycoprotein involved in apoptosis, cell death and complement activation, was found to be five-fold upregulated in the present study. Clusterin is a known potential biomarker involved in HCC metastasis [44–46]. Similarly, a glycated form of haptoglobin (>15-fold in HCC) has been shown to be elevated in sera from an HCC patient , indicating that quantitative proteomics coupled to lectin affinity method is a promising strategy for cancer biomarker study.
Novel HCC-associated Proteins Identified by Lectin Affinity Method
Fetuin (also called as alpha-2-HS-glycoprotein) is a glycoprotein present in serum, which is synthesized by hepatocytes and forms dimers. Although its exact function is unknown, it has been postulated to play a role in tissue growth and brain development. It is found to be a circulating inhibitor of vascular calcification . Fetuin has been shown to be associated with head and neck cancers . In our study, fetuin was overexpressed 3.8-fold in HCC. Alpha-2-glycoprotein 1, zinc-binding (ZAG) is a glycoprotein which has 18.2% carbohydrate and binds zinc ions. It is a secreted protein which stimulates lipid degradation in adipocytes . ZAG is not only expressed in normal liver and breast but also reported to be expressed in breast cancer [55, 56], prostate cancer [57, 58], and bladder cancer . In our study, alpha-2 glycoprotein 1 zinc was overexpressed 4.6-fold in HCC. Transgelin is an actin cross-linking protein found in fibroblasts and smooth muscles. Downregulation of transgelin expression in many cell lines may be a sensitive marker for the onset of transformation. It is reported to be overexpressed in breast cancer, cervical cancer, colorectal cancer, endometrial cancer, head and neck cancer, malignant lymphoma, ovarian cancer, pancreatic cancer, prostate cancer, skin cancer, stomach cancer, and urothelial cancer . Transgelin is reported to be an inhibitor of ARA54-enhanced androgen receptor transactivation which gives an insight into the suppressor role of transgelin in prostate cancer cell growth . In our study, transgelin was overexpressed 6.0-fold in HCC when compared to 2.5 in whole liver proteome without lectin affinity purification.
Destrin belongs to F-actin polymerizing factor family. It is ubiquitously expressed and reported to be associated with colon cancer  and Alzheimer’s disease . Destrin is overexpressed 6.5-fold in HCC with lectin affinity purification. Leucine-rich alpha-2 glycoprotein 1 belongs to a leucine-rich family of proteins which are involved in protein–protein interactions and signal transduction. This protein consists of a single polypeptide chain with one galactosamine and four glucosamine oligosaccharides attached. Leucine-rich alpha-2 glycoprotein 1 is reported to be a marker involved in neutrophilic granulocyte differentiation . It is also shown to be overexpressed in several cancers similar to transgelin . In our study, leucine-rich alpha-2 glycoprotein 1 was overexpressed 4.7-fold with lectin affinity purification. Heparan sulfate proteoglycan 2 or perlecan is a major component of basement membrane and has angiogenic and growth-promoting attributes. It acts as a co-receptor for basic fibroblasts growth factor. It is reported that antisense targeting of perlecan subsides tumor growth and angiogenesis, and could be a potential target for therapeutics ; it has been shown to be overexpressed in prostate cancer . In our study, perlecan was overexpressed 4.3-fold in HCC with lectin affinity enrichment. Hemopexin is a heme-binding plasma glycoprotein which is synthesized in liver and forms about 1.4% of total serum proteins. Each molecule of hemopexin binds to one heme and transports it to hepatocytes for salvage of the iron; it plays an important in receptor mediated cellular uptake of heme . It has been reported that expression of hemopexin is low in fetal liver and its expression increases in adult liver . In our study, hemopexin was overexpressed threefold in HCC.
Validation of CSRP1 and Fetuin Western Blot and IHC
Validation of Fetuin as an Overexpressed Protein in HCC Using Tissue Microarrays
Identification of N-glycosylation Sites
N-glycosylated peptides identified from lectin affinity enriched proteins of HCC tissue
RefSeq accession number
Clusterin isoform 1
Complement component 4A
Corticosteroid binding globulin
Fibrinogen, beta chain
Fibrinogen, gamma chain isoform gamma-A
Fibronectin 1 isoform 2
Fibronectin 1 isoform 2
FK506 binding protein 9
H factor (complement)-like 3
Inter-alpha globulin inhibitor H2
Kininogen 1 isoform 2
Leucine proline-enriched proteoglycan 1
Melanoma cell adhesion molecule
Microfibrillar-associated protein 4
Oxygen regulated protein
Serine (or cysteine) proteinase inhibitor, clade C
serpin peptidase inhibitor, clade A
Sex hormone-binding globulin
Levels of Transgelin and Destrin in Lectin-enriched Serum
Quantitative profiling of proteins in HCC revealed many overexpressed proteins relevant to HCC. Many proteins that have been previously associated with HCC were detected in this study. Important among those were complement component H, tenascin C, ceruloplasmin, haptoglobin, and alpha-2 macroglobin. More importantly, a number of novel HCC-associated proteins discovered in this study are candidates for validation in HCC using alternate platforms such as immunohistochemical labeling or ELISA. One of the novel candidates, fetuin was validated as a potential biomarker using tissue microarrays containing 114 tumor and 79 normal liver tissue sections. Although many lectin-bound proteins were found to be overexpressed in HCC, whole protein levels were also high in both normal and tumor cells. We also compared the extent of fold changes in proteins before and after lectin affinity enrichment to evaluate the status of glycosylation in HCC. Many of the proteins identified in this study showed differences in fold changes before and after lectin affinity enrichment indicating that this subset of proteins may represent hyperglycosylation associated with cancer. This approach is useful in identifying cancer-associated proteins which are otherwise undetectable in a complex mixture. For example, fetuin, SERPINA1, and destrin were identified only by lectin affinity method because of reduced complexity. Hemopexin, peptidyl prolyl isomerase B, cofilin 1, and HSP 27-kDa protein showed more fold changes in lectin affinity method indicating glycation specific changes in these proteins in HCC. Our study showed many N-linked glycosylation sites from HCC including novel sites, which may be of interest for further study of glycoprotein marker. Nearly 30% of the protein-bound lectins contain confirmed N-glycosylation sites indicating this technical approach of quantitative proteomics using multiple lectin affinity method can be used for simple screening of biomarkers in cancer. Majority of the confirmed N-glycosylation sites were found in proteins upregulated in HCC such as haptoglobin, hemopexin, clusterin, and ceruloplasmin. While many potential biomarker studies in HCC are based on identification and validation of single upregulated proteins which involve unfractionated serum or tissues, lectin affinity allows enrichment of subproteomes containing aberrantly glycated proteins and help in identification of multiple markers in a single analysis. A significant number of upregulated proteins (40%) identified in this study were also predicted to be N-glycosylated proteins . In our study, PNGase F treatment in the presence of 18O-water found to be useful for the identification of N-glycosylation sites in addition to the presence of consensus motif N-X-T/S. In conclusion, quantitative proteomic approach can be successfully used for the analysis of subproteomes, such as glycosylated proteins, to specifically identify and quantitate N-glycosylated proteins and peptides.
This work is supported by the family of Margaret Lee. A. P. is supported by National Institutes of Health Grant CA62924 and the Alexander and Margaret Stewart Trust and a grant from the Cancer Center (GI SPORE).
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