Fig. 3
From: Targeted Synovial Fluid Proteomics for Biomarker Discovery in Rheumatoid Arthritis
a Representative HPLC chromatogram for the first-dimension chromatofocusing separation of the abundant protein depleted SF sample. The diagonal line indicates the observed pH gradient. The fractions corresponding to the elution range of pH 5.63–5.45 from four runs were combined for the second-dimension separation. b HPLC chromatogram for the second-dimension reverse phase separation. This sample was separated on a Beckman System Gold HPLC coupled to a diode array detector. The diagonal line indicates the solvent gradient (%B). The fraction (between 20 and 25 min) corresponding to the region that tested positive in the protein array assay was selected for further characterization by mass spectrometry