Open Access

Erratum to: Characterization of Nitrotyrosine-Modified Proteins in Cerebrospinal Fluid

  • Ashley S. Beasley1,
  • Caroline Anderson2,
  • Justin McArthur2,
  • Ned Sacktor2,
  • Avindra Nath2, 3 and
  • Robert J. Cotter1Email author
Clinical Proteomics20106:9043

DOI: 10.1007/s12014-010-9043-2

Published: 15 April 2010

The original article was published in Clinical Proteomics 2010 6:9041

Erratum to: Clin Proteom

DOI 10.1007/s12014-010-9041-4

The original version of this article unfortunately missed the annotation for the oxidized methionines and nitrated tyrosines in Table 1 and on Figure 4, the images of the samples have appeared in thick black frames. The corrected Table 1 and Figure 4 are as follows
Table 1

Tryptic products of in vitro n-BSA

Peptide sequence

Charge

Peptide ID probability

Mascot Ion score

Mascot Identity score

X! Tandem -log(e) score

Observed m/z

Actual peptide mass (AMU)

Calculated +1H Peptide Mass (AMU)

Actual minus calculated peptide mass (AMU)

TVM* ENFVAFVDK

2

95.00%

71.6

38

5.02

708.3477

1,414.68

1,415.69

-0.0004417

IETM*REKVLTSSAR

3

64.20%

0

0

1.31

546.623

1,636.85

1,636.87

0.9842

DAFLGSFLYEY*SR

2

95.00%

41.8

38.3

5.48

806.8712

1,611.73

1,612.73

0.006302

LGEY*GFQNALIVR

2

95.00%

86.9

38.6

8.09

762.8956

1,523.78

1,524.78

0.002306

RHPEY*AVSVLLR

3

95.00%

47.5

38.8

4.57

495.6044

1,483.79

1,484.80

0.0003256

Y*LYEIAR

2

65.50%

29.8

36.6

0

486.7436

971.4716

972.4791

0.0004075

YLY*EIAR

2

68.90%

30.5

36.6

0

486.7436

971.4716

972.4791

0.0004075

Y*LY*EIAR

2

85.30%

35.6

36.5

0

509.2361

1,016.46

1,017.46

0.0003306

AEFVEVTK

2

95.00%

54.1

35.9

0

461.7474

921.4792

922.4886

-0.001529

AEFVEVTK

1

85.60%

43.3

35.9

0

922.4875

921.4797

922.4886

-0.001053

AEFVEVTKLVTDLTK

2

95.00%

0

0

3.44

847.9707

1,693.93

1,692.94

1.991

AFDEK

2

74.70%

29.8

34.4

0

305.1478

608.28

609.2885

-0.0006331

ATEEQLK

1

76.10%

27.9

36.3

0

818.4245

817.4167

818.4261

-0.001546

ATEEQLK

2

95.00%

51.1

36.3

0

409.7164

817.4172

818.4261

-0.001022

CCTKPESER

2

95.00%

64.7

36.6

1.48

526.7297

1,051.44

1,052.45

0.001065

CCTKPESER

3

95.00%

30.8

36.6

2

351.489

1,051.44

1,052.45

0.0008892

DAFLGSFLYEYSR

2

95.00%

79

38.3

8.03

784.3771

1,566.74

1,567.74

0.003179

DAFLGSFLYEYSRR

3

95.00%

26.8

45.6

1.74

575.2875

1,722.84

1,723.84

0.002598

DAIPENLPPLTADFAEDK

2

95.00%

50.8

46.3

5

978.4852

1,954.95

1,955.96

0.002055

DLGEEHFK

2

95.00%

51.1

36.6

0

487.7336

973.4516

974.4585

0.0009688

DTHKSEIAHR

3

95.00%

43.9

43.9

1.7

398.5388

1,192.59

1,193.60

-0.001902

FKDLGEEHFK

2

95.00%

59.3

37.6

3.72

625.3146

1,248.61

1,249.62

-0.0004376

FKDLGEEHFK

3

95.00%

59.6

37.6

3.96

417.2124

1,248.61

1,249.62

-0.0002136

FKDLGEEHFK

4

95.00%

27.5

37.5

1.29

313.1605

1,248.61

1,249.62

-0.00319

FPKAEFVEVTK

2

68.50%

0

0

1.85

648.3491

1,294.68

1,294.70

0.9857

FPKAEFVEVTK

3

95.00%

27.3

38.1

1.12

432.2374

1,293.69

1,294.70

-0.008115

FQNALLVR

2

93.50%

44.8

44

0

480.7861

959.5566

960.5631

0.001377

HLVDEPQNLIK

2

95.00%

52.2

38.1

5.66

653.3591

1,304.70

1,305.72

-0.006416

HLVDEPQNLIK

3

95.00%

39.1

38.1

1.27

435.9096

1,304.71

1,305.72

-0.003592

HPEYAVSVLLR

2

95.00%

40.1

38.1

2.08

642.3597

1,282.70

1,283.71

0.0004832

HPEYAVSVLLR

3

95.00%

47.3

38.1

3.68

428.5756

1,282.70

1,283.71

0.0001072

IETMR

1

58.40%

18.1

34.9

0

649.3345

648.3267

649.3344

0.0001521

IETMR

2

77.10%

31

34.9

0

325.1712

648.3268

649.3344

0.0002761

KQTALVELLK

2

95.00%

77

38.8

0

571.8612

1,141.71

1,142.72

-0.0004154

KQTALVELLK

3

95.00%

58.5

38.8

0

381.5762

1,141.71

1,142.72

-0.001991

KVPQVSTPTLVEVSR

2

95.00%

94.1

39.8

7.64

820.4717

1,638.93

1,639.94

-0.002713

KVPQVSTPTLVEVSR

3

95.00%

76.8

39.8

5.64

547.319

1,638.93

1,639.94

0.003111

LCVLHEK

2

95.00%

47

35.7

0

421.232

840.4484

841.4607

-0.004421

LGEYGFQNALIVR

2

95.00%

85.2

38.8

5.66

740.4017

1,478.79

1,479.80

-0.0004167

LSQKFPK

2

95.00%

43.7

36.5

0

424.2566

846.4976

847.5043

0.001177

LSQKFPKAEFVEVTK

3

95.00%

0

0

2.59

584.9897

1,751.95

1,750.97

1.979

LSQRFPK

2

93.40%

43.9

43.2

0

438.2521

874.4886

875.5104

-0.01393

LVNELTEFAK

1

95.00%

33.6

37.4

4.31

1,163.63

1,162.62

1,163.63

-0.001753

LVNELTEFAK

2

95.00%

74.4

37.4

2.82

582.3201

1,162.62

1,163.63

0.001171

HAGCEK

3

95.00%

34.7

40.1

2.54

832.05

2,493.13

2,494.16

-0.02511

LVTDLTK

1

79.70%

34.6

36.3

0

789.4715

788.4637

789.4724

-0.0008407

LVTDLTK

2

95.00%

53.2

36.3

0

395.2392

788.4628

789.4724

-0.001717

LVVSTQTALA

2

95.00%

68.3

37.2

3.44

501.795

1,001.57

1,002.58

-0.001308

QTALVELLK

2

95.00%

77.3

37.6

0

507.8132

1,013.61

1,014.62

-0.001414

RHPEYAVSVLLR

2

95.00%

0

0

4.51

720.9116

1,439.81

1,439.81

1.003

RHPEYAVSVLLR

3

95.00%

83.1

38.9

4.43

480.6088

1,438.80

1,439.81

-0.001398

SLHTLFGDELCK

2

95.00%

27.9

44.4

1.74

681.8406

1,361.67

1,362.67

0.000476

TCVADESHAGCEK

2

95.00%

66.3

37.7

6.1

675.2777

1,348.54

1,349.55

0.000973

TCVADESHAGCEK

3

95.00%

44

37.8

2.57

450.5207

1,348.54

1,349.55

-0.000103

TPVSEKVTK

2

95.00%

72.7

43.8

4.43

494.7871

987.5586

988.568

-0.001521

TVMENFVAFVDK

2

95.00%

70.1

38.1

3.43

700.3507

1,398.69

1,399.69

0.0004733

VASLR

2

78.80%

33.1

36.5

0

273.1742

544.3328

545.3411

-0.0004225

VPQVSTPTLVEVSR

2

95.00%

102

39.2

7.68

756.4266

1,510.84

1,511.84

0.002089

YLYEIAR

2

95.00%

45

36.3

0

464.2495

926.4834

927.494

-0.002716

M*- Oxidized Methionine

Y*- NO2-modified Tyrosine

Fig. 4

1-D electrophoresis (a) of nitrotyrosine enrichment fractions from pooled non-HIV (control) and HAND CSF. Lane 1, MW marker; lane 2, flowthrough (non-nitrated proteins); lane 3, pre-elution PBS wash; and lane 4, 5% formic acid elution (enriched nitrated proteins). The total number of spectra acquired (normalized spectrum count) from Non-HIV (control) and HAND CSF nitrotyrosine-modified subproteome (a). Quantitative immunoblot analysis of nitrotyrosine content in albumin/IgG-depleted non-HIV (control) and HAND (diseased) CSF (b) and L-PGDS (c) determined by slot blot analysis

Notes

Authors’ Affiliations

(1)
Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine
(2)
Neurology, Johns Hopkins University School of Medicine
(3)
Neuroscience, Johns Hopkins University School of Medicine

Copyright

© Springer Science+Business Media, LLC 2010

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