Fig. 2From: A Targeted Proteomics Approach for Biomarker Discovery Using Bilateral Matched Nipple Aspiration FluidsExpression pattern of cathepsin D detected by antibody array can be validated by western blot. Equal amounts of NAF protein (3 µg) were separated on a precast 4–12% SDS Bis–Tris gel. Cathepsin D was detected using monoclonal antibody from BD Biosciences. Samples from four patients and one control were studied. Single asterisk indicates tumor-associated NAF, triple plus signs indicate elevated expression detected by antibody array, broken line indicates decreased expression detected by antibody arrayBack to article page