Fig. 3

Immunoprecipitation followed by Western blot analysis was performed to confirm the carbonylation of C8A and IGKC proteins in plasma. a, b The efficiency of immunoprecipitation was checked with anti-C8A and anti-IGKC antibody (IP + WB), respectively. They were immunoprecipitated with respective antibodies and Western blot analysis with anti-DNP antibody (IP + DNP-derivate + WB). c, d Histograms represent the alteration of protein carbonyl levels in which the measured value is normalized with the mean of the control subjects. *p < 0.05 versus control