Table 2 Protein targets across the five multiplexed panels, showing allowable ranges of analytical parameters
Panel | Protein | Abbreviation | Dilution factor | Duplicate CV max (%) | Hill slope min | Hill slope max | LLoQ (pg/mL) | ULoQ (pg/mL) |
|---|---|---|---|---|---|---|---|---|
1 | Alpha-1-acid glycoprotein | A1AG | 300,000 | 20 | 0.9 | 1.1 | 12.21 | 50,000 |
1 | Alpha-1 antitrypsin | A1AT | 300,000 | 20 | 0.9 | 1.1 | 73.24 | 300,000 |
1 | Apolipoprotein A-I | APOA1 | 300,000 | 20 | 0.9 | 1.1 | 244.14 | 1,000,000 |
1 | Complement 3 | CO3 | 300,000 | 20 | 0.9 | 1.1 | 610.35 | 2,500,000 |
1 | Haptoglobin | HPT | 300,000 | 20 | 0.9 | 1.1 | 488.28 | 2,000,000 |
2 | Alpha-antichymotrypsin | AACT | 5000 | 20 | 0.6 | 0.8 | 1220.7 | 5,000,000 |
2 | Carbonic anhydrase 1 | CAH1 | 5000 | 20 | 0.9 | 1.1 | 2.44 | 10,000 |
2 | Clusterin | CLUS | 5000 | 20 | 0.8 | 1.2 | 244.14 | 1,000,000 |
2 | Complement 9 | CO9 | 5000 | 20 | 0.9 | 1.2 | 24.41 | 100,000 |
2 | C-reactive protein | CRP | 5000 | 20 | 0.9 | 1.1 | 12.21 | 50,000 |
2 | Dipeptidyl peptidase IV | DPPIV | 5000 | 20 | 0.9 | 1.1 | 2.44 | 10,000 |
2 | Serum amyloid A | SAA | 5000 | 20 | 0.9 | 1.1 | 12.21 | 50,000 |
2 | Transferrin receptor protein | TFRC | 5000 | 20 | 0.9 | 1.1 | 0.49 | 2000 |
3 | Protein S100-A8/-A9 | CALP | 100 | 20 | 1.1 | 1.6 | 48.83 | 200,000 |
3 | Cathepsin D | CATD | 100 | 20 | 0.9 | 1.1 | 19.53 | 80,000 |
3 | Growth differentiation factor 15 | GDF15 | 100 | 20 | 0.9 | 1.1 | 0.12 | 500 |
3 | Gelsolin | GELS | 100 | 20 | 0.85 | 1.1 | 488.28 | 2,000,000 |
3 | Prolyl endopeptidase FAP | SEPR | 100 | 20 | 0.85 | 1.15 | 2.44 | 10,000 |
3 | Tissue metalloproteinase inhibitor 1 | TIMP1 | 100 | 20 | 1.3 | 1.5 | 12.21 | 50,000 |
4 | Annexin A1 | ANXA1 | 4 | 20 | 0.9 | 1.1 | 12.21 | 50,000 |
4 | Carcinoembryonic antigen-related cell adhesion molecule 5 | CEA | 4 | 20 | 0.9 | 1.1 | 24.41 | 100,000 |
4 | Glycine-tRNA ligase | GARS | 4 | 20 | 0.9 | 1.1 | 122.07 | 500,000 |
4 | Macrophage migration inhibitory factor | MIF | 4 | 20 | 0.9 | 1.1 | 14.65 | 60,000 |
4 | Trefoil factor 3 | TFF3 | 4 | 20 | 0.9 | 1.1 | 0.49 | 2000 |
5 | Pyruvate kinase isozyme M2 | PKM2 | 4 | 20 | 0.9 | 1.2 | 7812.5 | 2,000,000 |
5 | Peroxiredoxin-1 | PRDX1 | 4 | 20 | 0.9 | 1.1 | 12.21 | 50,000 |
5 | P-selectin glycoprotein ligand 1 | PSGL | 4 | 20 | 0.9 | 1.1 | 12.21 | 50,000 |