Fig. 9From: iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failureQuantitative immunofluorescence of zona pellucida-binding protein 1 (ZPBP1) on the spermatozoa samples from the IVF pregnancy group (n = 20) and the R-ICSI pregnancy group (n = 20). IgG was used as negative control. PI, propidium iodide. Scale bar indicates 20 μm. Asterisk indicates significant difference analyzed by unpaired t-test (P < 0.05)Back to article page