Fig. 1
From: Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens
Evaluation setup and extraction mode. A Visual summary of the key aspects of FFPE protein extraction; B Dynamic ranges of the four marker proteins employed for western blot analysis; n = 2; C Exemplary image of a reaction chamber used for on-slide extraction. Triangles denote values outside of the visualization window; D On-slide extraction versus in-tube; n = 9, means ± standard deviation, normalized to median blot intensity to allow for visual variance comparison; E Comparison of the use of a thermocycler for heat-induced antigen retrieval compared to the standard protocol (identical temperature protocol); n = 5, medians with interquartile ranges of the respective data as whiskers