Table 3 Protein serum and plasma levels and ratio stability.
Protein | sNPXa | pNPXb | LOD NPXc | pNPX below LOD (%)d | sNPX below LOD (%)e | Mean ratiof | CV% ratiog | Olink within rangeh | Correla-tioni | Concentration effect j |
|---|---|---|---|---|---|---|---|---|---|---|
Adenosine deaminase (ADA) | 5.73 | 6.02 | 0.68 | 0 | 0 | 0,71 | 25,39 | Yes | 0.88 | No |
Adhesion G-protein coupled receptor G1 (ADGRG1) | 2.95 | 2.67 | 0.68 | 0 | 0 | 1,21 | 10,20 | Yes | 0.97 | No |
Angiopoietin-1 (ANGPT1) | 9.67 | 8.17 | − 0.01 | 0 | 0 | 2,50 | 29,41 | Yes | 0.72 | Yes |
Angiopoietin-2 (ANGPT2) | 6.87 | 6.78 | 0.33 | 0 | 0 | 1,13 | 19,77 | Yes | 0.88 | No |
Arginase-1 (ARG1) | 4.60 | 4.23 | 4.32 | 50 | 33.33 | 1,42 | 49,43 | Yes | 0.68 | No |
Carbonic anhydrase 9 (CAIX) | 6.14 | 5.87 | 1.04 | 0 | 0 | 1,20 | 5,69 | Yes | 0.99 | No |
Caspase-8 (CASP-8) | 4.45 | 5.35 | 1.56 | 0 | 0 | 0,57 | 37,88 | No | 0.34 | No |
C–C motif chemokine 17 (CCL17) | 10.93 | 10.19 | 1.35 | 0 | 0 | 1,47 | 40,64 | No | 0.70 | No |
C–C motif chemokine 19 (CCL19) | 11.73 | 11.77 | 1.40 | 0 | 0 | 1,06 | 12,44 | Yes | 0.92 | No |
C–C motif chemokine 20 (CCL20) | 8.58 | 9.18 | 1.12 | 0 | 0 | 0,62 | 16,25 | Yes | 0.98 | No |
C–C motif chemokine 23 (CCL23) | 11.39 | 11.21 | 0.61 | 0 | 0 | 1,12 | 10,98 | Yes | 0.99 | Yes |
C–C motif chemokine 3 (CCL3) | 7.65 | 7.05 | 0.48 | 0 | 0 | 1,42 | 14,97 | Yes | 0.73 | No |
C–C motif chemokine 4 (CCL4) | 7.61 | 6.95 | 0.52 | 0 | 0 | 1,64 | 20,66 | Yes | 0.81 | No |
Natural killer cell receptor 2B4 (CD244) | 6.93 | 7.17 | 1.67 | 0 | 0 | 0,76 | 22,67 | Yes | 0.70 | No |
CD27 antigen (CD27) | 8.74 | 8.67 | 0.63 | 0 | 0 | 1,07 | 6,35 | Yes | 0.93 | No |
T-cell-specific surface glycoprotein CD28 (CD28) | 0.87 | 0.76 | 0.29 | 8.33 | 0 | 1,07 | 12,70 | Yes | 0.81 | No |
T-cell surface glycoprotein CD4 (CD4) | 4.37 | 4.22 | 0.62 | 0 | 0 | 1,06 | 9,17 | No | 0.95 | No |
CD40L receptor (CD40) | 10.75 | 11.17 | 0.28 | 0 | 0 | 0,75 | 17,22 | No | 0.86 | No |
CD40 ligand (CD40-L) | 7.73 | 6.75 | 2.18 | 0 | 0 | 1,42 | 110,74 | No | 0.50 | No |
T-cell surface glycoprotein CD5 (CD5) | 6.41 | 6.39 | 0.56 | 0 | 0 | 1,03 | 6,61 | Yes | 0.91 | No |
CD70 antigen (CD70) | 4.54 | 3.77 | 0.51 | 0 | 0 | 1,71 | 10,19 | Yes | 0.90 | No |
CD83 antigen (CD83) | 3.24 | 3.11 | 0.44 | 0 | 0 | 1,06 | 9,06 | Yes | 0.80 | No |
T-cell surface glycoprotein CD8 alpha chain (CD8A) | 10.03 | 10.04 | 0.21 | 0 | 0 | 1,01 | 8,44 | Yes | 0.97 | No |
Cytotoxic and regulatory T-cell molecule (CRTAM) | 6.13 | 5.82 | 1.23 | 0 | 0 | 1,25 | 8,81 | Yes | 0.97 | No |
Macrophage colony-stimulating factor 1 (CSF-1) | 10.53 | 10.50 | 1.35 | 0 | 0 | 1,04 | 5,71 | Yes | 0.91 | No |
Fractalkine (CX3CL1) | 4.74 | 4.32 | 0.43 | 0 | 0 | 1,28 | 8,71 | Yes | 0.99 | No |
C-X-C motif chemokine 1 (CXCL1) | 10.35 | 10.31 | 0.99 | 0 | 0 | 1,04 | 61,37 | Yes | 0.70 | Yes |
C-X-C motif chemokine 10 (CXCL10) | 10.19 | 10.56 | 1.01 | 0 | 0 | 0,85 | 19,48 | Yes | 0.94 | No |
C-X-C motif chemokine 11 (CXCL11) | 9.59 | 10.37 | 0.77 | 0 | 0 | 0,61 | 51,33 | No | 0.76 | No |
Stromal cell-derived factor 1 (CXCL12) | 1.92 | 2.28 | 0.92 | 0 | 0 | 0,81 | 17,62 | Yes | 0.84 | No |
C-X-C motif chemokine 13 (CXCL13) | 9.81 | 9.60 | 1.43 | 0 | 0 | 1,08 | 23,29 | Yes | 0.94 | Yes |
C-X-C motif chemokine 5 (CXCL5) | 12.59 | 12.37 | 1.39 | 0 | 0 | 1,07 | 48,26 | No | 0.59 | No |
C-X-C motif chemokine 9 (CXCL9) | 8.31 | 8.47 | 0.76 | 0 | 0 | 0,96 | 10,60 | Yes | 0.80 | No |
Decorin (DCN) | 4.89 | 4.52 | 0.92 | 0 | 0 | 1,30 | 8,46 | Yes | 0.66 | No |
Pro-epidermal growth factor (EGF) | 9.28 | 8.45 | 0.69 | 0 | 0 | 1,29 | 123,45 | No | 0.36 | No |
Tumor necrosis factor ligand superfamily member 6 (FASLG) | 7.07 | 7.05 | 0.69 | 0 | 0 | 1,05 | 8,75 | Yes | 0.92 | No |
Fibroblast growth factor 2 (FGF2)# | 0.43 | 2.74 | 0.53 | 8.33 | 75 | NA | NA | NA | NA | NA |
Galectin-1 (Gal-1) | 7.08 | 7.11 | 1.19 | 0 | 0 | 0,96 | 8,27 | Yes | 0.88 | No |
Galectin-9 (Gal-9) | 8.80 | 8.78 | 0.24 | 0 | 0 | 1,03 | 5,89 | Yes | 0.92 | No |
Granzyme A (GZMA) | 7.47 | 7.35 | 0.53 | 0 | 0 | 1,05 | 19,82 | Yes | 0.74 | No |
Granzyme B (GZMB) | 3.40 | 4.01 | 0.28 | 0 | 0 | 0,79 | 53,05 | Yes | 0.57 | No |
Granzyme H (GZMH) | 4.56 | 4.76 | 1.46 | 0 | 0 | 0,89 | 19,02 | Yes | 0.88 | No |
Hepatocyte growth factor (HGF) | 10.22 | 9.45 | 0.65 | 0 | 0 | 1,71 | 26,41 | Yes | 0.86 | No |
Heme oxygenase 1 (HO-1) | 12.66 | 12.50 | 4.41 | 0 | 0 | 1,04 | 8,04 | Yes | 0.97 | No |
ICOS ligand (ICOSLG) | 6.25 | 5.74 | 0.39 | 0 | 0 | 1,40 | 7,82 | Yes | 0.83 | No |
Interferon gamma (IFN-gamma)* | 7.82 | 7.64 | 1.92 | 0 | 0 | 1,07 | 9,81 | NA | 0.99 | No |
Interleukin-1 alpha (IL-1 alpha)# | 0.22 | 0.48 | 2.24 | 100 | 100 | 0,95 | 36,09 | NA | NA | NA |
Interleukin-10 (IL10) | 4.49 | 4.39 | 1.67 | 0 | 0 | 1,15 | 13,41 | Yes | 0.95 | No |
Interleukin-12 (IL12) | 7.59 | 7.44 | 1.50 | 0 | 0 | 1,12 | 10,68 | Yes | 0.95 | No |
Interleukin-12 receptor subunit beta-1 (IL12RB1) | 2.52 | 2.49 | 1.26 | 0 | 0 | 1,05 | 12,94 | Yes | 0.90 | No |
Interleukin-13 (IL13)# | 1.63 | 1.60 | 2.49 | 91.67 | 91.67 | NA | NA | NA | NA | NA |
Interleukin-15 (IL15)* | 5.43 | 5.29 | 2.29 | 0 | 0 | 1,12 | 9,18 | NA | 0.91 | No |
Interleukin-18 (IL18) | 10.05 | 10.20 | 1.16 | 0 | 0 | 0,95 | 18,08 | Yes | 0.93 | No |
Interleukin-2 (IL2)# | 1.35 | 1.30 | 2.27 | 100 | 100 | NA | NA | NA | NA | NA |
Interleukin-33 (IL33)# | − 0.57 | − 0.42 | − 0.06 | 91.67 | 100 | NA | NA | NA | NA | NA |
Interleukin-4 (IL4) | 1.05 | 1.36 | 1.08 | 16.67 | 50 | 0,86 | 28,44 | Yes | 0.36 | No |
Interleukin-5 (IL5)# | 1.03 | 0.99 | 1.44 | 66.67 | 75 | NA | NA | NA | NA | NA |
Interleukin-6 (IL6) | 4.82 | 4.87 | 1.05 | 0 | 0 | 1,08 | 10,86 | Yes | 1.00 | No |
Interleukin-7 (IL7) | 6.80 | 6.13 | 0.71 | 0 | 0 | 1,62 | 47,62 | Yes | 0.53 | No |
Interleukin-8 (IL8) | 8.07 | 6.99 | 2.24 | 0 | 0 | 2,70 | 37,77 | Yes | 0.92 | No |
Killer cell immunoglobulin-like receptor 3DL1 (KIR3DL1)* | 2.46 | 2.50 | 1.29 | 0 | 8.33 | 0,98 | 16,28 | NA | 0.96 | No |
Natural killer cells antigen CD94 (KLRD1) | 7.19 | 7.03 | 0.25 | 0 | 0 | 1,16 | 6,72 | Yes | 0.99 | No |
Lymphocyte activation gene 3 protein (LAG3)* | 5.81 | 5.50 | 1.05 | 0 | 0 | 1,22 | 7,69 | NA | 0.97 | No |
Lysosome-associated membrane glycoprotein 3 (LAMP3) | 5.38 | 5.26 | 0.66 | 0 | 0 | 1,14 | 7,93 | Yes | 0.97 | No |
Latency-associated peptide transforming growth factor beta-1 (LAP TGF-beta-1) | 10.26 | 10.10 | 1.67 | 0 | 0 | 1,01 | 26,78 | No | 0.87 | No |
C–C motif chemokine 2 (MCP-1) | 12.01 | 11.24 | 0.72 | 0 | 0 | 1,92 | 20,32 | No | 0.80 | No |
C–C motif chemokine 8 (MCP-2) | 8.96 | 8.38 | 0.79 | 0 | 0 | 1,51 | 23,85 | Yes | 0.76 | No |
C–C motif chemokine 7 (MCP-3) | 3.12 | 2.26 | 0.91 | 0 | 0 | 1,86 | 21,03 | Yes | 0.71 | No |
C–C motif chemokine 13 (MCP-4) | 11.19 | 10.57 | 0.39 | 0 | 0 | 1,53 | 35,37 | Yes | 0.53 | No |
MHC class I polypeptide-related sequence A/B (MIC-A/B) | 6.16 | 6.03 | 0.78 | 0 | 0 | 1,07 | 7,49 | Yes | 0.93 | No |
Macrophage metalloproteinase-12 (MMP12) | 8.97 | 8.43 | 0.42 | 0 | 0 | 1,31 | 20,91 | Yes | 0.97 | Yes |
Matrix metalloproteinase-7 (MMP7) | 13.04 | 11.49 | 4.09 | 0 | 0 | 3,01 | 19,69 | No | 0.55 | No |
Mucin-16 (MUC-16)* | 5.59 | 5.52 | 0.20 | 0 | 0 | 1,04 | 8,40 | NA | 0.99 | No |
Natural cytotoxicity triggering receptor (NCR1) | 4.09 | 4.16 | 1.34 | 0 | 0 | 1,10 | 13,09 | Yes | 0.89 | No |
Nitric oxide synthase, endothelial (NOS3) | 2.55 | 3.34 | 1.97 | 0 | 16.67 | 0,63 | 30,82 | Yes | 0.72 | No |
Programmed cell death protein 1 (PDCD1) | 5.67 | 5.65 | 2.53 | 0 | 0 | 1,00 | 8,44 | Yes | 0.96 | No |
Platelet-derived growth factor subunit B (PDGF subunit B) | 10.65 | 10.38 | 0.73 | 0 | 0 | 1,26 | 28,30 | Yes | -0.52 | Yes |
Programmed cell death 1 ligand 1 (PD-L1) | 6.74 | 7.03 | 1.85 | 0 | 0 | 0,84 | 13,84 | Yes | 0.93 | No |
Programmed cell death 1 ligand 2 (PD-L2) | 3.72 | 3.61 | 0.93 | 0 | 0 | 1,13 | 6,24 | Yes | 0.96 | No |
Placenta growth factor (PGF) | 9.17 | 9.09 | 0.75 | 0 | 0 | 1,09 | 7,17 | Yes | 0.94 | No |
Pleiotrophin (PTN) | 1.47 | 2.93 | 1.14 | 0 | 33.33 | 0,41 | 41,52 | Yes | 0.44 | No |
Angiopoietin-1 receptor (TIE2) | 7.77 | 7.65 | 0.54 | 0 | 0 | 1,05 | 6,87 | Yes | 0.97 | No |
Tumor necrosis factor (TNF)* | 5.23 | 5.20 | 2.28 | 0 | 0 | 1,00 | 12,36 | NA | 0.87 | No |
Tumor necrosis factor receptor superfamily member 12A (TNFRSF12A) | 6.33 | 6.13 | -0.10 | 0 | 0 | 1,27 | 6,94 | No | 0.99 | No |
Tumor necrosis factor receptor superfamily member 21 (TNFRSF21) | 7.86 | 7.77 | -0.39 | 0 | 0 | 1,10 | 6,39 | Yes | 0.95 | No |
Tumor necrosis factor receptor superfamily member 4 (TNFRSF4) | 6.89 | 6.78 | 0.82 | 0 | 0 | 1,13 | 8,21 | Yes | 0.96 | No |
Tumor necrosis factor receptor superfamily member 9 (TNFRSF9) | 7.44 | 7.19 | 0.81 | 0 | 0 | 1,13 | 5,58 | Yes | 0.99 | No |
Tumor necrosis factor ligand superfamily member 14 (TNFSF14) | 7.07 | 6.20 | 0.70 | 0 | 0 | 2,07 | 39,87 | Yes | 0.80 | No |
TNF-related apoptosis-inducing ligand (TRAIL) | 8.48 | 8.12 | 0.53 | 0 | 0 | 1,32 | 10,56 | Yes | 0.73 | No |
Tumor necrosis factor ligand superfamily member 12 (TWEAK) | 8.58 | 8.20 | -0.06 | 0 | 0 | 1,46 | 23,29 | Yes | 0.64 | No |
Vascular endothelial growth factor A (VEGFA) | 10.80 | 10.33 | 0.37 | 0 | 0 | 1,45 | 24,07 | Yes | 0.93 | No |
Vascular endothelial growth factor receptor 2 (VEGFR-2) | 8.20 | 8.12 | 0.54 | 0 | 0 | 1,07 | 6,52 | Yes | 0.92 | No |