Fig. 3

Quantification of CA3 (A) and LDHB (B) using immuno-based sandwich bead arrays. Measurements of protein standards are represented by open circles whereas those of patient samples with closed circles. Only samples with MFI values falling between the upper and lower limits were considered quantifiable. The lower limit was defined as samples with MFI values at least two standard deviations above the mean background MFI. The upper limit was defined as samples with MFI values of 90% or lower of the MFI value of the most concentrated recombinant protein standard sample. The gray horizontal lines denote the mean MFI value of all blank samples, with dotted lines indicating one standard deviation above and below the mean background MFI. Standard curves were estimated using five parametric log-logistic regression (5PL)