Table 1 Amino Acid Sequences of SARS-CoV-2 Nucleoprotein (Upper Panel) and Tryptic Peptides (Lower Panel). Amino acids corresponding to peptides (A-D) that were used for the MALDI-TOF-MS assay are printed in bold. Peptide D was detected in two variants (D377 and Y377). Peptides A and D were additionally used in their extended forms (ext) with one missed cleavage site of trypsin; peptides A and C were also employed as stable isotope-labeled peptides (SIL) with C-terminal arginine residues labeled (marked as R*)

SARS-CoV-2 Nucleoprotein

MSDNGPQNQR¹⁰ NAPRITFGGP²⁰ SDSTGSNQNG³⁰ ERSGARSKQR⁴⁰ RPQGLPNNTA⁵⁰ SWFTALTQHG⁶⁰ KEDLKFPRGQ⁷⁰GVPINTNSSP⁸⁰DDQIGYYRRA⁹⁰ TRRIRGGDGK¹⁰⁰ MKDLSPRWYF¹¹⁰ YYLGTGPEAG¹²⁰ LPYGANKDGI¹³⁰ IWVATEGALN¹⁴⁰ TPKDHIGTRN¹⁵⁰PANNAAIVLQ¹⁶⁰LPQGTTLPKG¹⁷⁰ FYAEGSRGGS¹⁸⁰ QASSRSSSRS¹⁹⁰ RNSSRNSTPG²⁰⁰ SSRGTSPARM²¹⁰ AGNGGDAALA²²⁰ LLLLDRLNQL²³⁰ ESKMSGKGQQ²⁴⁰ QQGQTVTKKS²⁵⁰ AAEASKKPRQ²⁶⁰ KRTATKAYNV²⁷⁰TQAFGRRGPE²⁸⁰ QTQGNFGDQE²⁹⁰ LIRQGTDYKH³⁰⁰ WPQIAQFAPS³¹⁰ ASAFFGMSRI³²⁰ GMEVTPSGTW³³⁰ LTYTGAIKLD³⁴⁰ DKDPNFKDQV³⁵⁰ ILLNKHIDAY³⁶⁰ KTFPPTEPKK³⁷⁰ DKKKKADETQ³⁸⁰ALPQRQKKQQ³⁹⁰ TVTLLPAADL⁴⁰⁰ DDFSKQLQQS⁴¹⁰ MSSADSTQA

Peptide

Amino Acid Sequence

Position

Isotope Labeling