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Table 1 A review of previous processing protocols used for proteomic analysis of saliva

From: Proteomic Studies of Saliva: A Proposal for a Standardized Handling of Clinical Samples

Ref.

Saliva type

Temperature of collection

Addition of protease inhibitor

Removal of unsoluble material

Temperature of samples storage

Proteomic analysis

54

Stimulated parotid saliva

Ice

No

No

−20°C

HPLC and N-terminal sequencing

20

Whole saliva

Unknown

No

No

−20°C

SDS-PAGE and MALDI-TOF

21

Stimulated glandular and whole saliva

Ice

No

Centrifugation

−20°C

2DE and MALDI-TOF

22

Stimulated whole saliva

Ice

No

Centrifugation

No storage: direct analysis

2DE and MALDI-TOF

23

Unstimulated whole saliva

Unknown

No

Centrifugation

Unknown

2DE and MALDI-TOF-TOF

26

Unstimulated whole saliva

Ice

Yes

Centrifugation

−20°C

2DE and MALDI-TOF, Q-TOF, N-terminal sequencing and Western blotting

25

Whole saliva

Unknown

No

Centrifugation

No storage: direct analysis

HPLC and LC-MS and MALDI-TOF

55

Unstimulated whole saliva

Unknown

No

Centrifugation

Unknown

2D LC-MS

27

Stimulated parotid saliva

Ice

No

Centrifugation

−20°C

2DE and MALDI-TOF MALDI-TOF-TOF and Q-TOF

29

Whole saliva

Ice

Yes

Centrifugation

−80°C

Shotgun proteomics and 2DE and MALDI-TOF

30

Stimulated whole saliva

Ice

Yes

Centrifugation

−80°C

2DE and MALDI-TOF

33

Unstimulated whole saliva

Unknown

No

Centrifugation

Unknown

FFE and LC-MS/MS

31

Stimulated whole saliva

Ice

Yes

Centrifugation

−80°C

2DE and MALDI-TOF

32

Stimulated whole saliva

Ice

Yes

Centrifugation

−80°C

2DE and MALDI-TOF

34

Stimulated whole and parotid saliva

Unknown

No

No

−80°C

2DE and MALDI-TOF and LC-MS/MS

35

Unstimulated whole saliva

Unknown

No

Centrifugation

−80°C

2DE and MALDI-TOF

36

Parotid saliva

Ice

No

Centrifugation

−80°C

2D-DIGE and SELDI-TOF

37

Unstimulated whole saliva, parotid

Ice

No

Filtration

−80°C

2DE and MALDI-TOF

38

Unstimulated whole saliva

Ice

No

Centrifugation

No storage: direct analysis

2DE and MALDI-TOF

39

Stimulated and unstimulated whole saliva

Unknown

Yes

Centrifugation

Ice, −20°C and −80°C

SELDI-TOF

  1. MS = mass spectrometry, SELDI = surface enhanced laser desorption/ionization