From: Proteomic Studies of Saliva: A Proposal for a Standardized Handling of Clinical Samples
Ref. | Saliva type | Temperature of collection | Addition of protease inhibitor | Removal of unsoluble material | Temperature of samples storage | Proteomic analysis |
---|---|---|---|---|---|---|
Stimulated parotid saliva | Ice | No | No | −20°C | HPLC and N-terminal sequencing | |
Whole saliva | Unknown | No | No | −20°C | SDS-PAGE and MALDI-TOF | |
Stimulated glandular and whole saliva | Ice | No | Centrifugation | −20°C | 2DE and MALDI-TOF | |
Stimulated whole saliva | Ice | No | Centrifugation | No storage: direct analysis | 2DE and MALDI-TOF | |
Unstimulated whole saliva | Unknown | No | Centrifugation | Unknown | 2DE and MALDI-TOF-TOF | |
Unstimulated whole saliva | Ice | Yes | Centrifugation | −20°C | 2DE and MALDI-TOF, Q-TOF, N-terminal sequencing and Western blotting | |
Whole saliva | Unknown | No | Centrifugation | No storage: direct analysis | HPLC and LC-MS and MALDI-TOF | |
Unstimulated whole saliva | Unknown | No | Centrifugation | Unknown | 2D LC-MS | |
Stimulated parotid saliva | Ice | No | Centrifugation | −20°C | 2DE and MALDI-TOF MALDI-TOF-TOF and Q-TOF | |
Whole saliva | Ice | Yes | Centrifugation | −80°C | Shotgun proteomics and 2DE and MALDI-TOF | |
Stimulated whole saliva | Ice | Yes | Centrifugation | −80°C | 2DE and MALDI-TOF | |
Unstimulated whole saliva | Unknown | No | Centrifugation | Unknown | FFE and LC-MS/MS | |
Stimulated whole saliva | Ice | Yes | Centrifugation | −80°C | 2DE and MALDI-TOF | |
Stimulated whole saliva | Ice | Yes | Centrifugation | −80°C | 2DE and MALDI-TOF | |
Stimulated whole and parotid saliva | Unknown | No | No | −80°C | 2DE and MALDI-TOF and LC-MS/MS | |
Unstimulated whole saliva | Unknown | No | Centrifugation | −80°C | 2DE and MALDI-TOF | |
Parotid saliva | Ice | No | Centrifugation | −80°C | 2D-DIGE and SELDI-TOF | |
Unstimulated whole saliva, parotid | Ice | No | Filtration | −80°C | 2DE and MALDI-TOF | |
Unstimulated whole saliva | Ice | No | Centrifugation | No storage: direct analysis | 2DE and MALDI-TOF | |
Stimulated and unstimulated whole saliva | Unknown | Yes | Centrifugation | Ice, −20°C and −80°C | SELDI-TOF |