Proteomic Studies of Saliva: A Proposal for a Standardized Handling of Clinical Samples

Table 2 Identification of the 30 most abundant spots from the two-dimensional gel of whole saliva (see Fig. 2), by peptide mass fingerprint MALDI-TOF

No	Group	Protein name	Ref.	% Cov.	MW (kDa) theoretical	Observed	pI theoretical	Observed
1	A	Serum albumin	P02768	29	66.5	68	5.67	5.5
2	A	Serum albumin	P02768	25	66.5	68	5.67	5.6
3	A	Serum albumin	P02768	25	66.5	68	5.67	5.8
4	A	Serum albumin	P02768	27	66.5	68	5.67	6
5	A	Serum albumin	P02768	25	66.5	68	5.67	6.2
6	F	Salivary-amylase	P04745	42	55.9	59	6.34	5.5
7	F	Salivary-amylase	P04745	33	55.9	59	6.34	5.8
8	F	Salivary-amylase	P04745	28	55.9	59	6.34	6
9	F	Salivary-amylase	P04745	51	55.9	59	6.34	6.1
10	F	Salivary-amylase	P04745	50	55.9	59	6.34	6.3
11	F	Salivary-amylase	P04745	51	55.9	59	6.34	6.5
12	F	Salivary-amylase	P04745	50	55.9	59	6.34	6.7
13	G	Zinc-alpha-2-glycoprotein	P25311	52	32.1	42	5.58	5.1
14	G	Zinc-alpha-2-glycoprotein	P25311	53	32.1	42	5.58	5.3
15	G	Zinc-alpha-2-glycoprotein	P25311	57	32.1	42	5.58	5.5
16	G	Zinc-alpha-2-glycoprotein	P25311	53	32.1	42	5.58	5.7
17	B	Salivary acidic PRP	P02810	36	15.4	26	4.63	4.1
18	B	Salivary acidic PRP	P02810	42	15.4	25	4.63	4.2
19	C	Prolactin inducible	P12273	38	13.5	18	5.47	4.2
20	C	Prolactin inducible	P12273	50	13.5	18	5.47	4.5
21	C	Prolactin inducible	P12273	43	13.5	18	5.47	5
22	C	Salivary acidic protein 1	P01036	27	14.2	16	4.83	4.2
23	C	Salivary acidic protein 1	P01036	29	14.2	16	4.83	4.4
24	C	Salivary acidic protein 1	P01036	34	14.2	16	4.83	4.7
25	D	Salivary cystatin SA-1	P01037	40	14.3	16	6.92	7
26	E	Salivary-amylase	P04745	34	55.9	30	6.34	6.4
27	E	Salivary-amylase	P04745	22	55.9	35	6.34	6.5
28	E	Salivary-amylase	P04745	35	55.9	30	6.34	6.7
29	E	Salivary-amylase	P04745	43	55.9	28	6.34	6.8
30	E	Salivary-amylase	P04745	27	55.9	27	6.34	7.1

Protein reference (Ref.) corresponds to the Swiss-Prot/NCBI accession number; sequence coverage (% cov.) is given as percentage

ISSN: 1559-0275