Table 3 Stability of saliva proteins according to the one-dimension and two-dimensional electrophoresis experiments
From: Proteomic Studies of Saliva: A Proposal for a Standardized Handling of Clinical Samples
Temperature of samples storage | Duration of storage | Addition of protease inhibitor | Removal of unsoluble material | Degradation of proteinsa | Protein groups sensibleb |
|---|---|---|---|---|---|
20°C | 0 | − | + | − | None |
20°C | 10 min | − | + | − | / |
20°C | 60 min | − | + | − | / |
20°C | 1 day | − | + | +/− | A |
20°C | 7 days | − | + | + | A, B, C, D, G |
20°C | 30 days | − | + | + | A, B, C, D, G |
20°C | 30 days | + | + | + | A, B, C, D, G |
20°C | 30 days | − | − | +++ | / |
4°C | 10 min | − | + | − | / |
4°C | 60 min | − | + | − | / |
4°C | 1 day | − | + | − | / |
4°C | 7 days | − | + | +/− | / |
4°C | 30 days | − | + | +/− | / |
4°C | 30 days | + | + | − | / |
4°C | 30 days | − | − | +++ | / |
−20°C | 30 days | − | + | − | / |
−80°C | 30 days | − | + | − | / |