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Fig. 3 | Clinical Proteomics

Fig. 3

From: Liquid Chromatography/Mass Spectrometry (LC/MS)-Based Glycoproteomics Technologies for Cancer Biomarker Discovery

Fig. 3

Identification and structural analysis of glycopeptides. Glycopeptides eluted from LC is introduced into MS through an electrospray interface. CID of the precursor ion produces fragment ions dissociated preferentially at glycosyl bond. The MS3 fragments of peptide provide amino acid sequence information assigned by database search tools, and the MS3 fragments of glycan moeties provide structural information on the glycan attached on the glycopeptide. Alternatively, ECD/ETD of the glycopeptide precursor ion cleaves preferentially at peptide bonds and provides the amino acid sequence and a glycosylated amino acid with an additional mass of attached glycans. Thus, the detailed glycan structure can be determined by concurrent measurement with CID-MSn

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