Table 1 Lectins used for glycopeptide capture and their binding specificity for glycans
Lectin | Source | Specificity |
|---|---|---|
ConA | Canavalia ensiformis | α-Man (no binding in the presence of bisecting GlcNAc) |
GNA | Galanthus nivalis | Non-substituted a1–6Man |
MAL | Maackia amurensis | Siaα 2–3Galβ1–4Glc(NAc) |
SSA | Sambucus sieboldiana | Siaα 2–6 Galβ1–4Glc(NAc) |
MAH | Maackia amurensis | Siaα2–3Gal |
WGA | Triticum unlgari | Multivalent Sia and (GlcNAc)n |
LTL | Lotus tetragonolobus | Fucα1–3GlcNAc, Sia-Lex, and Lex |
PHA-E | Phaseolus vulgaris | Bisecting GlcNAc and biantennary N-glycans |
GSL-II | Griffonia simplicifolia | GlcNAc and agalactosylated N-glycans |
LCA | Lens culinaris | Fucα1–6GlcNAc (core fucose) |
UEA-I | Ulex europaeus | Fucα1–2 Galβ1–4Glc(NAc) |
AOL | Aspergillus oryzae | Fucose |
AAL | Aleuria aurantia | Fucose |
LEL | Lycopersicon esculentum | Poly-LacNAc and (GlcNAc)n |
DSA | Datura stramonium | Poly-LacNAc and branched LacNAc |
ECA | Erythrina cristagalli | Galβ1–4Glc(NAc) |
RCA120 | Ricinus communis | Galβ1–4Glc(NAc) |
Jacalin | Artocarpus integrifolia | Galβ1–3GalNAcα-Ser/Thr (T) and GalNAcα-Ser/Thr (Tn) |
PNA | Arachis hypogaea | Galβ1–3GalNAcα-Ser/Thr (T) |
WFA | Wisteria floribunda | Terminal GalNAc (e.g., GalNAcβ1–4GlcNAc) |