Fig. 3
From: Kinetic Analysis of Glycoprotein–Lectin Interactions by Label-Free Internal Reflection Ellipsometry
a–d Binding of four glycoproteins to 14 lectins or lectin combinations after 4-min incubation and 15 min rinse with running buffer to remove non-specific binding. The lectins are arranged in ascending order in terms of the binding to fetuin, and the same order was repeated for the remaining graphs. The substrate represents areas of the surface not containing lectin and is a measure of non-specific binding. Mixtures are given by three numbers separated by colons and represent the concentration ratio of ConA/WGA/jacalin within each microarray spot normalized to ConA concentration in milligrams per milliliter. M-LAC is an equimolar ratio of the three lectins