Skip to main content
Fig. 3 | Clinical Proteomics

Fig. 3

From: Glycomic Approaches to Study GlcNAcylation: Protein Identification, Site-mapping, and Site-specific O-GlcNAc Quantitation

Fig. 3

Chemoenzymatic approaches for O-GlcNAc peptide enrichment. A Full scan spectra of GlcNAcylated peptide (TAPTgSTIAPG, [M+GlcNAc+H]+ 1118.5) solution before and after 12-h incubation with Y289L GalT1 and UDP-azido-Gal. B Flow charts representing approaches using different functional groups for biotinylation [18, 35, 36]. TCEP, Tris(2-carboxyethyl)phosphine hydrochloride; TBTA, Tris[(1-benzyl-1H-1,2,3-triazol-4-yl)methyl]amine

Back to article page

Clinical Proteomics

ISSN: 1559-0275