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Fig. 1 | Clinical Proteomics

Fig. 1

From: 18O Labeling for a Quantitative Proteomic Analysis of Glycoproteins in Hepatocellular Carcinoma

Fig. 1

Strategy used for the enrichment of glycosylated proteins for 16O/18O labeling based quantitative mass spectrometric analysis of liver proteins in HCC. Tissue lysates (4 mg) were prepared from tumor and non-tumor liver tissues from a diagnosed case of HCC and incubated with mixture of lectins. The lectin-bound proteins were eluted using mixture of sugars and bound proteins derived from tumor and non-tumor samples were resolved separated on SDS-PAGE. In-gel trypsin digestion was carried out in the presence of 16O/18O-water differential labeling of peptides derived from non-tumor/tumor samples. After mixing the labeled and unlabeled peptides, the LC-MS/MS analysis was done using quadrupole time-of-flight mass spectrometer

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