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Table 3 N-glycosylated peptides identified from lectin affinity enriched proteins of HCC tissue

From: 18O Labeling for a Quantitative Proteomic Analysis of Glycoproteins in Hepatocellular Carcinoma

RefSeq accession number Protein name Expect value Mascot score Peptide sequence Known? Consensus motif
NP_000005.2 Alpha-2-macroglobulin 1.0E−01 42 SLGNV*NFTVSAEALESQELCGTEVPSVPEHGRK YES NFT
2.1E−03 57 VS*NQTLSLFFTVLQDVPVR YES NQT
NP_000375 Apolipoprotein B 8.3E−05 62 F*NSSYLQGTNQITGR YES NSS
NP_001638.1 Apolipoprotein D 7.2E−02 42 ADGTVNQIEGEATPV*NLTEPAKLEVK YES NLT
P00450 Ceruloplasmin 3.4E−06 85 E*NLTAPGSDSAVFFEQGTTR YES NLT
NP_001822.2 Clusterin isoform 1 2.1E−05 76 LA*NLTQGEDQYYLR YES NLT
NP_009224.2 Complement component 4A 1.3E−02 47 GL*NVTLSSTGR NO NVT
NP_001747.2 Corticosteroid binding globulin 3.4E−04 54 AQLLQGLGF*NLTER YES NLT
NP_005132.2 Fibrinogen, beta chain 9.5E−05 59 GTAGNALMDGASQLMGE*NR(T) NO NRT
NP_000500.2 Fibrinogen, gamma chain isoform gamma-A 1.5E−06 78 DLQSLEDILHQVE*NK(T) YES NKT
NP_006673.1 Fibrinogen-like 2 9.9E−02 38 LDGST*NFTR NO NFT
7.1E−05 62 LHVGNY*NGTAGDALR NO NGT
NP_997640.1 Fibronectin 1 isoform 2 4.3E−02 44 DQCIVDDITYNV*NDTFHK YES NDT
NP_997640 Fibronectin 1 isoform 2 8.9E−07 82 LDAPTNLQFV*NETDSTVLVR NO NET
NP_009201 FK506 binding protein 9 1.1E−03 51 YHYNASLLDGTLLDSTWNLGK NO NAS
NP_005657.1 H factor (complement)-like 3 6.5E−05 61 LQNNEN*NISCVER NO NIS
NP_005134 Haptoglobin 1.5E−05 78 MVSHH*NLTTGATLINEQWLLTTAK YES NLT
3.5E−07 85 NLFL*NHSENATAK YES NHS
2.9E−06 71 VVLHP*NYSQVDIGLIK YES NYS
NP_000604 Hemopexin 2.1E−05 76 ALPQPQ*NVTSLLGCTH YES NVT
3.5E−08 94 SWPAVG*NCSSALR YES NCS
NP_000403.1 Histidine-rich glycoprotein 5.6E−03 52 VIDF*NCTTSSVSSALANTK YES NCT
NP_002207 Inter-alpha globulin inhibitor H2 2.3E−02 37 GAFISNFSMTVDGK YES NFS
NP_000884 Kininogen 1 isoform 2 5.4E−06 73 ITYSIVQTNCSK NO NCS
NP_071751 Leucine proline-enriched proteoglycan 1 2.7E−03 47 VPLQSAHLYYNVTEK NO NVT
NP_006491 Melanoma cell adhesion molecule 7.2E−03 42 CVASVPSIPGLNR(T) NO NRT
NP_002395 Microfibrillar-associated protein 4 2.3E−04 57 FNGSVSFFR NO NGS
1.1E−04 69 VDLEDFE*NNTAYAK NO NNT
NP_006380.1 Oxygen regulated protein 3.2E−09 104 LSALDNLL*NHSSMFLK YES NHS
CAJ15161 Alpha-antitrypsin 4.0E−08 102 YLG*NATAIFFLPDEGK YES NAT
NP_000479.1 Serine (or cysteine) proteinase inhibitor, clade C 8.1E−06 70 LGAC*NDTLQQLMEVFK YES NDT
NP_001076.2 serpin peptidase inhibitor, clade A 2.3E−04 65 F*NLTETSEAEIHQSFQHLLR NO NLT
NP_001031.2 Sex hormone-binding globulin 9.5E−03 41 LDVDQAL*NR(S) NO NRS
NP_001054 Transferrin 7.4E−06 72 CGLVPVLAENY*NK(S) YES NKS
3.0E−02 46 QQQHLFGS*NVTDCSGNFCLFR YES NVT
  1. Accession number and protein names were from NCBI RefSeq database, and mascot score and expect values were listed for N-glycosylated peptides. Human protein reference database (www.hprd.org) was used for the identification of previously reported N-glycosylation sites. An asterisk in *N-X-T/S implies 18O labeling of Asn after PNGase F mediated cleavage of glycan.