Fig. 1

Total protein and glycoprotein-stained 2-D gels. Whole saliva was subjected to isoelectric focusing on a pH 3–10 nonlinear IPG strip, which was then resolved in the second dimension on a 12.5% SDS-PAGE gel. The resulting gels were either stained with SYPRO Ruby total protein stain (a) or Pro-Q Emerald 488 glycoprotein stain (b). Labeled are the protein spots that were identified from the Sypro Ruby-stained gel either by MALDI-TOF MS or LC-MS/MS in our previous work [8] or by LC-MS/MS in this work