Glycoprofiling of the Human Salivary Proteome

Table 2 Cross-reactivity table

Primary specificity	Fucose		Galactose		Mannose and fucose	Mannose and glucose	Mannose		N-Acetyl- galactosamine		N-Acetyl- glucosamine			Sialic acid		Glycols
Lectin or stain	AAL	UEA I	JAC	MAL I	PSA	ConA	GNL	HHL	BPL	VVA	DSL	LEL	WGA	MAL II	SNA	PQE
AAL	54	45	39	49	43	45	43	44	46	48	41	30	42	46	42	49
UEA I	45	63	36	54	41	54	57	51	57	50	53	47	44	52	44	46
JAC	39	36	59	48	35	43	45	52	50	46	38	25	36	50	41	39
MAL I	49	54	48	109	47	63	69	82	79	76	58	39	55	97	61	66
PSA	43	41	35	47	54	48	44	46	48	44	46	33	29	46	43	51
ConA	45	54	43	63	48	74	63	62	65	57	47	41	56	65	52	60
GNL	43	57	45	69	44	63	91	75	74	66	56	45	53	80	56	58
HHL	44	51	52	82	46	62	75	109	79	75	54	38	53	93	64	64
BPL	46	57	50	79	48	65	74	79	101	75	59	50	54	85	62	70
VVA	48	50	46	76	44	57	66	75	75	95	55	40	52	63	61	68
DSL	41	53	38	58	46	47	56	54	59	55	69	40	54	42	50	44
LEL	30	47	25	39	33	41	45	38	50	40	40	78	39	40	36	38
WGA	42	44	36	55	29	56	53	53	54	52	54	39	62	53	53	54
MAL II	46	52	50	97	46	65	80	93	85	63	42	40	53	128	60	74
SNA	42	44	41	61	43	52	56	64	62	61	50	36	53	60	70	56
PQE	49	46	39	66	51	60	58	64	70	68	44	38	54	74	56	94

This table demonstrates the number of spots showing cross-reactivity between two lectins or a lectin and the Pro-Q Emerald 488 stain (PQE). Spots showing only low, medium, or high reactivity were counted. Data in bold are the number of spots from a total of 166 spots, which reacted with a particular lectin or was stained with Pro-Q Emerald

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