Fig. 2

Effect of high-fat feeding on glucose tolerance. Glucose tolerance was measured by intraperitoneally injecting a 25% glucose solution at 0.01 mL/g body weight after 4 h of fasting. Blood glucose was measured immediately before the glucose injection (0 min) and at 30, 60, and 90 min after the injection by tail bleeding using the ONE TOUCH glucometer from Lifescan (Milpitas, CA, USA). Average blood glucose levels of high-fat fed group (n = 10) and control group (n = 5) at 60 min after glucose injection were plotted against time on high-fat diet. In order to double the number of blood collections while minimizing the stress of bleeding on the mice, each of control (n = 10) and high-fat fed (n = 20) cohort was divided into two bleeding groups, and blood was collected every other week from each group until 8 weeks. Then blood collection was continued at the indicated time points by alternating between the bleeding groups. Error bars represent the SEM. Statistical analysis was performed using ANOVA. Single asterisk indicates p < 0.05