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Fig. 3 | Clinical Proteomics

Fig. 3

From: Plasma Protein Biomarkers Correlated with the Development of Diet-Induced Type 2 Diabetes in Mice

Fig. 3

Effect of high-fat feeding on plasma insulin levels. Average plasma insulin concentrations of control (n = 5) and high-fat fed (n = 10) mice were plotted for the duration on the high-fat diet. Mice were fasted for 4 h and approximately 200 µL of tail blood was collected in heparinized capillary tubes and plasma was separated by centrifugation at 7,000 × g for 10 min at 4°C and stored at −80°C. A part of the plasma samples was used to quantify insulin levels using an ultrasensitive rat/mouse insulin ELISA kit (ALPCO, Windham, NH, USA). Fasting plasma insulin levels were higher in high-fat fed mice except at 4-week time point and the highest level was observed between 8 and 13 weeks. Error bars represent the SEM. Statistical analysis was performed using ANOVA. Single asterisk indicates p < 0.05

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