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Fig. 5 | Clinical Proteomics

Fig. 5

From: Plasma Protein Biomarkers Correlated with the Development of Diet-Induced Type 2 Diabetes in Mice

Fig. 5

Quantity changes of RBP4 isoforms. Locations of RBP4 isoforms are indicated by circles in the partial image of two-dimensional gel described in Fig. 4 and numbered 1 to 4 from acidic to basic. Average fluorescence intensity of an indicated isoform is plotted for control (n = 5) and high-fat fed (n = 8) mice. Plasma sample from each mouse was resolved on a two-dimensional gel, and image was captured using a laser-scanner (Pharos FX plus, Bio-Rad) with the excitation–emission setting at 473–520 nm. Detection, matching, and quantification of each protein spot were performed using the PDQuest 7.0.1 program (Bio-Rad) assuming loading of equal amount of total protein per gel. All of RBP4 spots were cut out from the polyacrylamide gel and analyzed by MS/MS at the Michigan Proteome Consortium. Concentration of total RBP4 (top right panel) was estimated as a sum of fluorescence intensities of all isoforms. Error bars represent the SEM. Statistical analysis was performed using ANOVA. Single asterisk indicates p < 0.05

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