Fig. 3
From: Characterization of Nitrotyrosine-Modified Proteins in Cerebrospinal Fluid
LTQ-Orbitrap analysis of in vitro n-BSA tryptic peptides: RHPEYAVSVLLR and RHPEYNO2AVSVLLR. The base peak ion chromatograms of the MS analysis of the tryptic digestion of in vitro n-BSA are presented in Panel A. The single ion chromatograms at m/z 480.61 and m/z 495.60 represent the detection of the [M+3H]+3 ion of the non-nitrated and nitrated BSA peptide, respectively. The tandem MS spectra of m/z 480.61 and m/z 495.60 are in Panel C. In addition to changing the peptide mass, the –NO2 group also increases the Tyrosine immonium ion from m/z 136.10 to m/z 181.06 (Panel B)