Table 3 Identified peptides from tryptic digest of in vivo nitrated L-PGDS from non-HIV (control) and HAND CSF
From: Characterization of Nitrotyrosine-Modified Proteins in Cerebrospinal Fluid
Peptide sequence | Charge | Mascot Ion Score | Observed m/z | Actual peptide mass (AMU) | Calculated +1H peptide mass (AMU) | Actual minus calculated peptide mass (AMU) |
|---|---|---|---|---|---|---|
Non-HIV (n = 3) | ||||||
AQGFTEDTIVFLPQTDK | 2 | 54 | 955.5187 | 1,909.0228 | 1,908.9469 | 0.0759 |
EKFTAFCKAQGFTEDTIVFLPQTDK | 3 | 35 | 955.5182 | 2,863.5328 | 2,863.4102 | 0.1225 |
KNQCETRTM*LLQPAGSLGSYSYR | 3 | 30 | 873.8182 | 2,618.4328 | 2,618.2581 | 0.1747 |
SPHWGSTYSVSVVETDYDQYALLYS QGSK | 2 | 41 | 1,634.174 | 3,266.3334 | 3,266.5044 | –0.171 |
SVVAPATDGGLNLTSTFLR | 2 | 132 | 960.0486 | 1,918.0826 | 1,918.016 | 0.0666 |
TM*LLQPAGSLGSYSYR | 2 | 68 | 881.0337 | 1,760.0528 | 1,758.8611 | 1.1918 |
WFSAGLASNSSWLR | 2 | 20 | 791.1036 | 1,580.1926 | 1,580.7736 | –0.5809 |
HAND (n = 4) | ||||||
AQGFTEDTIVFLPQTDK | 2 | 77 | 955.4286 | 1,908.8426 | 1,908.9469 | –0.1043 |
EKFTAFCKAQGFTEDTIVFLPQTDK | 3 | 42 | 955.4282 | 2,863.2628 | 2,863.4102 | –0.1475 |
KNQCETRTM*LLQPAGSLGSYSYR | 3 | 17 | 874.1215 | 2,619.3427 | 2,618.2581 | 1.0846 |
SPHWGSTYSVSVVETDYDQYALLYS QGSK | 3 | 74 | 1,090.261 | 3,267.7612 | 3,266.5044 | 1.2568 |
SVVAPATDGGLNLTSTFLR | 2 | 129 | 959.8787 | 1,917.7428 | 1,918.016 | –0.2732 |
TMLLQPAGSLGSYSYR | 2 | 92 | 872.3737 | 1,742.7328 | 1,742.8661 | –0.1333 |
TMLLQPAGSLGSY*SYR | 2 | 50 | 894.941 | 1,787.88 | 1,788.874 | –0.994 |
WFSAGLASNSSWLR | 2 | 20 | 791.4687 | 1,580.9228 | 1,580.7736 | 0.1493 |