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Fig. 2 | Clinical Proteomics

Fig. 2

From: A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

Fig. 2

Changes in proteins with increasing time to breast tumor freezing. Ten human breast tumors were collected immediately at surgery and frozen after increasing time intervals up until 24 h. Of the nine total and phosphoproteins shown as examples, three showed a progressive increase with increasing time to breast tumor freezing (cleaved caspase 7, cleaved PARP, and phosphorylation of S6 at serines 235/236 (S6p235–236)), three showed a progressive deterioration with increasing time to breast tumor freezing (phosphorylation of AMP-activated protein kinase (AMPKp), MAPK (MAPKp), and src (srcp527)) and three did not change with increasing time to breast tumor freezing up to 24 h (4EBP1 expression and phosphorylation (4EBP1p37) and Akt expression). The mean expression of each total and phosphoprotein across the ten tumors relative to the mean expression level at time 0 was expressed in log2 units on the y-axis of each plot (with 95% confidence intervals (CI) also shown) and the series of times until breast tumor tissue freezing is shown on the x-axis of each plot (0, 0.5, 1, 2, 4, 6, and 24 h)

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