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Fig. 7 | Clinical Proteomics

Fig. 7

From: A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

Fig. 7

Reverse phase protein array (RPPA) reproducibility for four antibodies. Five serial 2-fold dilutions were made from 48 protein lysates (experimental outline shown in Fig. 8) and the serial dilutions were spotted in triplicate on three sets of nitrocellulose-coated slides at two time points separated by 1 month (“technical replicates”) followed by probing of each slide set with four antibodies to determine intraslide, interslide, and interbatch reproducibility, respectively. The individual correlation coefficients (R) for pairs of replicates for intraslide, interslide, and interbatch reproducibility are shown under each correlation plot for each antibody

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