Skip to main content
Fig. 8 | Clinical Proteomics

Fig. 8

From: A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

Fig. 8

Experimental outline for the reverse phase protein array (RPPA) reproducibility experiment in Fig. 7. Five serial 2-fold dilutions were made from 48 protein lysates, and the serial dilutions were spotted in triplicate on three sets of nitrocellulose-coated slides at two time points separated by 1 month, followed by probing of each slide set with four antibodies to determine intraslide, interslide, and interbatch reproducibility for the total and phosphoproteins detected by these four antibodies

Back to article page