Fig. 8

Experimental outline for the reverse phase protein array (RPPA) reproducibility experiment in Fig. 7. Five serial 2-fold dilutions were made from 48 protein lysates, and the serial dilutions were spotted in triplicate on three sets of nitrocellulose-coated slides at two time points separated by 1 month, followed by probing of each slide set with four antibodies to determine intraslide, interslide, and interbatch reproducibility for the total and phosphoproteins detected by these four antibodies