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Table 1 Eighty-two monospecific antibodies used in this study

From: A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

Antibody name

Protein name

Companya

cat#

Host

Dilution

4EBP1

4E Binding Protein 1

Cell Signaling Technology, Inc.

CS 9452

Rabbit

1 in 100

4EBP1p37

4EBP1 phosphorylation at T37/T46

Cell Signaling Technology, Inc.

CS 9459

Rabbit

1 in 100

AcCoA

Acetyl CoA Carboxylase

Epitomics, Inc.

1768-1

Rabbit

1 in 250

AcCoAp

AcCoA phosphorylation at S79

Cell Signaling Technology, Inc.

CS 3661

Rabbit

1 in 250

Akt

Protein Kinase B

Cell Signaling Technology, Inc.

CS 9272

Rabbit

1 in 250

Aktp308

Akt phosphorylation at S308

Cell Signaling Technology, Inc.

CS 9275

Rabbit

1 in 250

Aktp473

Akt phosphorylation at S473

Cell Signaling Technology, Inc.

CS 9271

Rabbit

1 in 250

AMPK

AMPK

Cell Signaling Technology, Inc.

CS 2532

Rabbit

1 in 250

AMPKp

AMPK phosphorylation at S172

Cell Signaling Technology, Inc.

CS 2535

Rabbit

1 in 250

β catenin

B catenin

Cell Signaling Technology, Inc.

CS 9562

Rabbit

1 in 300

bcl2

bcl2

Dako

M0887

Mouse

1 in 200

BRCA1

BRCA1

Upstate Biotechnology, Inc.

07-434

Rabbit

1 in 1,000

Caveolin 1

Caveolin 1

Cell Signaling Technology, Inc.

CS 3232

Rabbit

1 in 250

CCNB1

Cyclin B1

Epitomics, Inc.

1495-1

Rabbit

1 in 500

CCND1

Cyclin D1

Santa Cruz Biotechnology, Inc.

SC-718

Rabbit

1 in 1,000

CCNE1

Cyclin E1

Santa Cruz Biotechnology, Inc.

SC-247

Mouse

1 in 500

CD31

CD31

Dako

M0823

Mouse

1 in 500

CDK4

CDK4

Cell Signaling Technology, Inc.

CS 2906

Rabbit

1 in 250

cjun

Cjun

Cell Signaling Technology, Inc.

CS 9165

Rabbit

1 in 250

ckit

Ckit

Cell Signaling Technology, Inc.

 

Rabbit

1 in 150

cleaved caspase 7

Cleaved caspase 7 (Asp198)

Cell Signaling Technology, Inc.

CS 9491

Rabbit

1 in 150

cleaved PARP

Cleaved PARP (Asp214)

Cell Signaling Technology, Inc.

CS 9546

Mouse

1 in 250

cmyc

Cmyc

Cell Signaling Technology, Inc.

CS 9402

Rabbit

1 in 150

Collagen VI

Collagen VI

Santa Cruz Biotechnology, Inc.

SC-20649

Rabbit

1 in 750

COX2

COX2

Epitomics, Inc.

2,169-1

Rabbit

1 in 500

E cadherin

E cadherin

Cell Signaling Technology, Inc.

CS 4065

Rabbit

1 in 200

EGFR

Epidermal growth factor receptor

Santa Cruz Biotechnology, Inc.

SC-03

Rabbit

1 in 200

EGFRp1045

EGFR phosphorylation at Y1045

Cell Signaling Technology, Inc.

CS 2237

Rabbit

1 in 100

EGFRp922

EGFR phosphorylation at Y992

Cell Signaling Technology, Inc.

CS 2235

Rabbit

1 in 100

ER

Estrogen receptor alpha

Lab Vision Corporation (formerly Neomarkers)

Sp1

Rabbit

1 in 250

ERK2

Mitogen-activated protein kinase

Cell Signaling Technology, Inc.

SC-154

Rabbit

1 in 250

ERp118

ER phosphorylation at S118

Epitomics, Inc.

1091-1

Rabbit

1 in 200

ERp167

ER phosphorylation at S167

Epitomics, Inc.

2492-1

Rabbit

1 in 200

GSK3

Glycogen synthase kinase 3 beta

Santa Cruz Biotechnology, Inc.

SC-7291

Mouse

1 in 1,000

GSK3p21_9

GSK3 phosphorylation at S21/S9

Cell Signaling Technology, Inc.

CS 9331

Rabbit

1 in 250

HER2

Human epidermal receptor 2

Epitomics, Inc.

1148-1

Rabbit

1 in 250

HER2p1248

HER2 phosphorylation at Y1248

Upstate Biotechnology, Inc.

06-229

Rabbit

1 in 750

IGF1R

Insulin-like growth factor receptor 1

Cell Signaling Technology, Inc.

CS 3027

Rabbit

1 in 500

IGFRp

IGF1R phosphorylation at Y1135/Y1136

Cell Signaling Technology, Inc.

CS 3024

Rabbit

1 in 200

JNK

cjun N terminal Kinase

Santa Cruz Biotechnology, Inc.

SC-474

Rabbit

1 in 200

JNKp183-185

JNK phosphorylation at T183/Y185

Cell Signaling Technology, Inc.

CS 9251

Rabbit

1 in 150

MAPKp

MAPK1/2 phosphorylation at T202/T204

Cell Signaling Technology, Inc.

CS 4377

Rabbit

1 in 1,000

MEK1

MAPK/ERK kinase 1

Epitomics, Inc.

1235-1

Rabbit

1 in 15,000

MEK12p

MEK1/2 phosphorylation at T217/T221

Cell Signaling Technology, Inc.

CS 9121

Rabbit

1 in 800

mTOR

mammalian target of rapamycin

Cell Signaling Technology, Inc.

CS 2983

Rabbit

1 in 400

p110alpha

p110alpha subunit of phosphatidylinositol-3-kinase

Epitomics, Inc.

1683-1

Rabbit

1 in 500

p21

p21

Santa Cruz Biotechnology, Inc.

SC-397

Rabbit

1 in 250

p27

p27

Santa Cruz Biotechnology, Inc.

SC-527

Rabbit

1 in 500

p38

p38 MAPK

Cell Signaling Technology, Inc.

CS 9212

Rabbit

1 in 300

p38p180_2

p38 MAPK phosphorylation at T180/T182

Cell Signaling Technology, Inc.

CS 9211

Rabbit

1 in 250

p53

p53

Cell Signaling Technology, Inc.

CS 9282

Rabbit

1 in 3,000

p7056 Kinase

p70S6 Kinase

Epitomics, Inc.

1494-1

Rabbit

1 in 500

p70S6Kp389

p70S6 Kinase phosphorylation at T389

Cell Signaling Technology, Inc.

CS 9205

Rabbit

1 in 200

PAI1

Plasminogen activator inhibitor-1

BD Biosciences

612024

Mouse

1 in 1,000

pcmyc

cmyc phosphorylation at T58/S62

Cell Signaling Technology, Inc.

CS 9401

Rabbit

1 in 150

PDK1

Phosphoinositide-dependent kinase 1

Cell Signaling Technology, Inc.

CS 3062

Rabbit

1 in 250

PDK1p241

PDK1 phosphorylation at S241

Cell Signaling Technology, Inc.

CS 3061

Rabbit

1 in 500

PKCalpha

Protein Kinase C alpha

Upstate Biotechnology, Inc.

05-154

Mouse

1 in 2,000

PKCaphap657

PKCalpha phosphorylation at S657

Upstate Biotechnology, Inc.

06-822

Rabbit

1 in 3,000

pmTOR

mTOR phosphorylation at S2448

Cell Signaling Technology, Inc.

CS 2971

Rabbit

1 in 150

PR

Progesterone receptor

Epitomics, Inc.

1483-1

Rabbit

1 in 400

PTEN

PTEN

Cell Signaling Technology, Inc.

CS 9552

Rabbit

1 in 500

Rab25

Rab25

Courtesy Dr. Kwai Wa Cheng, MDACC

Covance

Rabbit

1 in 4,000

Rb

Retinoblastoma

Cell Signaling Technology, Inc.

CS 9309

Mouse

1 in 3,000

Rbp

Rb phosphorylation at S807/S811

Cell Signaling Technology, Inc.

CS 9308

Rabbit

1 in 250

S6

S6 ribosomal protein

Cell Signaling Technology, Inc.

CS 2217

Rabbit

1 in 200

S6p235–236

S6 phosphorylation at S235/S236

Cell Signaling Technology, Inc.

CS 2211

Rabbit

1 in 3,000

S6p240_4

S6 phosphorylation at S240/S244

Cell Signaling Technology, Inc.

CS 2215

Rabbit

1 in 3,000

SGK

Serum Glucocorticoid Kinase

Cell Signaling Technology, Inc.

CS 3272

Rabbit

1 in 250

SGKp

SGK phosphorylation at S78

Cell Signaling Technology, Inc.

CS 3271

Rabbit

1 in 250

src

Src

Upstate Biotechnology, Inc.

05-184

Mouse

1 in 200

srcp416

src phosphorylation at Y416

Cell Signaling Technology, Inc.

CS 2101

Rabbit

1 in 150

srcp527

src phosphorylation at Y527

Cell Signaling Technology, Inc.

CS 2105

Rabbit

1 in 400

stat3

Signal transducer and activator of transcription 3

Upstate Biotechnology, Inc.

06-596

Rabbit

1 in 500

stat3p705

stat3 phosphorylation at S705

Cell Signaling Technology, Inc.

CS 9131

Rabbit

1 in 500

stat3p727

stat3 phosphorylation at S727

Cell Signaling Technology, Inc.

CS 9134

Rabbit

1 in 250

stat6p641

stat6 phosphorylation at Y641

Cell Signaling Technology, Inc.

CS 9361

Rabbit

1 in 150

stathmin

Stathmin

Epitomics, Inc.

1972-1

Rabbit

1 in 500

TSC2

Tuberous Sclerosis Kinase 2

Epitomics, Inc.

1613-1

Rabbit

1 in 500

TSC2p

TSC2 phosphorylation at T1462

Cell Signaling Technology, Inc.

CS 3617

Rabbit

1 in 200

VEGFR2

KDR2/VEGF Receptor 2

Cell Signaling Technology, Inc.

CS 2479

Rabbit

1 in 700

XIAP

X linked inhibitor of apoptosis

Cell Signaling Technology, Inc.

CS 2042

Rabbit

1 in 200

  1. aCompanies—Abcam, Inc. (Cambridge, MA), BD Biosciences (San Jose, CA), Cell Signaling Technology, Inc. (Danvers, MA), Dako (Carpinteria, CA), Epitomics, Inc. (Burlingame, CA), Santa Cruz Biotechnology, Inc. (Santa Cruz, CA), Upstate Biotechnology, Inc. (Millipore)