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Table 1 Eighty-two monospecific antibodies used in this study

From: A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

Antibody name Protein name Companya cat# Host Dilution
4EBP1 4E Binding Protein 1 Cell Signaling Technology, Inc. CS 9452 Rabbit 1 in 100
4EBP1p37 4EBP1 phosphorylation at T37/T46 Cell Signaling Technology, Inc. CS 9459 Rabbit 1 in 100
AcCoA Acetyl CoA Carboxylase Epitomics, Inc. 1768-1 Rabbit 1 in 250
AcCoAp AcCoA phosphorylation at S79 Cell Signaling Technology, Inc. CS 3661 Rabbit 1 in 250
Akt Protein Kinase B Cell Signaling Technology, Inc. CS 9272 Rabbit 1 in 250
Aktp308 Akt phosphorylation at S308 Cell Signaling Technology, Inc. CS 9275 Rabbit 1 in 250
Aktp473 Akt phosphorylation at S473 Cell Signaling Technology, Inc. CS 9271 Rabbit 1 in 250
AMPK AMPK Cell Signaling Technology, Inc. CS 2532 Rabbit 1 in 250
AMPKp AMPK phosphorylation at S172 Cell Signaling Technology, Inc. CS 2535 Rabbit 1 in 250
β catenin B catenin Cell Signaling Technology, Inc. CS 9562 Rabbit 1 in 300
bcl2 bcl2 Dako M0887 Mouse 1 in 200
BRCA1 BRCA1 Upstate Biotechnology, Inc. 07-434 Rabbit 1 in 1,000
Caveolin 1 Caveolin 1 Cell Signaling Technology, Inc. CS 3232 Rabbit 1 in 250
CCNB1 Cyclin B1 Epitomics, Inc. 1495-1 Rabbit 1 in 500
CCND1 Cyclin D1 Santa Cruz Biotechnology, Inc. SC-718 Rabbit 1 in 1,000
CCNE1 Cyclin E1 Santa Cruz Biotechnology, Inc. SC-247 Mouse 1 in 500
CD31 CD31 Dako M0823 Mouse 1 in 500
CDK4 CDK4 Cell Signaling Technology, Inc. CS 2906 Rabbit 1 in 250
cjun Cjun Cell Signaling Technology, Inc. CS 9165 Rabbit 1 in 250
ckit Ckit Cell Signaling Technology, Inc.   Rabbit 1 in 150
cleaved caspase 7 Cleaved caspase 7 (Asp198) Cell Signaling Technology, Inc. CS 9491 Rabbit 1 in 150
cleaved PARP Cleaved PARP (Asp214) Cell Signaling Technology, Inc. CS 9546 Mouse 1 in 250
cmyc Cmyc Cell Signaling Technology, Inc. CS 9402 Rabbit 1 in 150
Collagen VI Collagen VI Santa Cruz Biotechnology, Inc. SC-20649 Rabbit 1 in 750
COX2 COX2 Epitomics, Inc. 2,169-1 Rabbit 1 in 500
E cadherin E cadherin Cell Signaling Technology, Inc. CS 4065 Rabbit 1 in 200
EGFR Epidermal growth factor receptor Santa Cruz Biotechnology, Inc. SC-03 Rabbit 1 in 200
EGFRp1045 EGFR phosphorylation at Y1045 Cell Signaling Technology, Inc. CS 2237 Rabbit 1 in 100
EGFRp922 EGFR phosphorylation at Y992 Cell Signaling Technology, Inc. CS 2235 Rabbit 1 in 100
ER Estrogen receptor alpha Lab Vision Corporation (formerly Neomarkers) Sp1 Rabbit 1 in 250
ERK2 Mitogen-activated protein kinase Cell Signaling Technology, Inc. SC-154 Rabbit 1 in 250
ERp118 ER phosphorylation at S118 Epitomics, Inc. 1091-1 Rabbit 1 in 200
ERp167 ER phosphorylation at S167 Epitomics, Inc. 2492-1 Rabbit 1 in 200
GSK3 Glycogen synthase kinase 3 beta Santa Cruz Biotechnology, Inc. SC-7291 Mouse 1 in 1,000
GSK3p21_9 GSK3 phosphorylation at S21/S9 Cell Signaling Technology, Inc. CS 9331 Rabbit 1 in 250
HER2 Human epidermal receptor 2 Epitomics, Inc. 1148-1 Rabbit 1 in 250
HER2p1248 HER2 phosphorylation at Y1248 Upstate Biotechnology, Inc. 06-229 Rabbit 1 in 750
IGF1R Insulin-like growth factor receptor 1 Cell Signaling Technology, Inc. CS 3027 Rabbit 1 in 500
IGFRp IGF1R phosphorylation at Y1135/Y1136 Cell Signaling Technology, Inc. CS 3024 Rabbit 1 in 200
JNK cjun N terminal Kinase Santa Cruz Biotechnology, Inc. SC-474 Rabbit 1 in 200
JNKp183-185 JNK phosphorylation at T183/Y185 Cell Signaling Technology, Inc. CS 9251 Rabbit 1 in 150
MAPKp MAPK1/2 phosphorylation at T202/T204 Cell Signaling Technology, Inc. CS 4377 Rabbit 1 in 1,000
MEK1 MAPK/ERK kinase 1 Epitomics, Inc. 1235-1 Rabbit 1 in 15,000
MEK12p MEK1/2 phosphorylation at T217/T221 Cell Signaling Technology, Inc. CS 9121 Rabbit 1 in 800
mTOR mammalian target of rapamycin Cell Signaling Technology, Inc. CS 2983 Rabbit 1 in 400
p110alpha p110alpha subunit of phosphatidylinositol-3-kinase Epitomics, Inc. 1683-1 Rabbit 1 in 500
p21 p21 Santa Cruz Biotechnology, Inc. SC-397 Rabbit 1 in 250
p27 p27 Santa Cruz Biotechnology, Inc. SC-527 Rabbit 1 in 500
p38 p38 MAPK Cell Signaling Technology, Inc. CS 9212 Rabbit 1 in 300
p38p180_2 p38 MAPK phosphorylation at T180/T182 Cell Signaling Technology, Inc. CS 9211 Rabbit 1 in 250
p53 p53 Cell Signaling Technology, Inc. CS 9282 Rabbit 1 in 3,000
p7056 Kinase p70S6 Kinase Epitomics, Inc. 1494-1 Rabbit 1 in 500
p70S6Kp389 p70S6 Kinase phosphorylation at T389 Cell Signaling Technology, Inc. CS 9205 Rabbit 1 in 200
PAI1 Plasminogen activator inhibitor-1 BD Biosciences 612024 Mouse 1 in 1,000
pcmyc cmyc phosphorylation at T58/S62 Cell Signaling Technology, Inc. CS 9401 Rabbit 1 in 150
PDK1 Phosphoinositide-dependent kinase 1 Cell Signaling Technology, Inc. CS 3062 Rabbit 1 in 250
PDK1p241 PDK1 phosphorylation at S241 Cell Signaling Technology, Inc. CS 3061 Rabbit 1 in 500
PKCalpha Protein Kinase C alpha Upstate Biotechnology, Inc. 05-154 Mouse 1 in 2,000
PKCaphap657 PKCalpha phosphorylation at S657 Upstate Biotechnology, Inc. 06-822 Rabbit 1 in 3,000
pmTOR mTOR phosphorylation at S2448 Cell Signaling Technology, Inc. CS 2971 Rabbit 1 in 150
PR Progesterone receptor Epitomics, Inc. 1483-1 Rabbit 1 in 400
PTEN PTEN Cell Signaling Technology, Inc. CS 9552 Rabbit 1 in 500
Rab25 Rab25 Courtesy Dr. Kwai Wa Cheng, MDACC Covance Rabbit 1 in 4,000
Rb Retinoblastoma Cell Signaling Technology, Inc. CS 9309 Mouse 1 in 3,000
Rbp Rb phosphorylation at S807/S811 Cell Signaling Technology, Inc. CS 9308 Rabbit 1 in 250
S6 S6 ribosomal protein Cell Signaling Technology, Inc. CS 2217 Rabbit 1 in 200
S6p235–236 S6 phosphorylation at S235/S236 Cell Signaling Technology, Inc. CS 2211 Rabbit 1 in 3,000
S6p240_4 S6 phosphorylation at S240/S244 Cell Signaling Technology, Inc. CS 2215 Rabbit 1 in 3,000
SGK Serum Glucocorticoid Kinase Cell Signaling Technology, Inc. CS 3272 Rabbit 1 in 250
SGKp SGK phosphorylation at S78 Cell Signaling Technology, Inc. CS 3271 Rabbit 1 in 250
src Src Upstate Biotechnology, Inc. 05-184 Mouse 1 in 200
srcp416 src phosphorylation at Y416 Cell Signaling Technology, Inc. CS 2101 Rabbit 1 in 150
srcp527 src phosphorylation at Y527 Cell Signaling Technology, Inc. CS 2105 Rabbit 1 in 400
stat3 Signal transducer and activator of transcription 3 Upstate Biotechnology, Inc. 06-596 Rabbit 1 in 500
stat3p705 stat3 phosphorylation at S705 Cell Signaling Technology, Inc. CS 9131 Rabbit 1 in 500
stat3p727 stat3 phosphorylation at S727 Cell Signaling Technology, Inc. CS 9134 Rabbit 1 in 250
stat6p641 stat6 phosphorylation at Y641 Cell Signaling Technology, Inc. CS 9361 Rabbit 1 in 150
stathmin Stathmin Epitomics, Inc. 1972-1 Rabbit 1 in 500
TSC2 Tuberous Sclerosis Kinase 2 Epitomics, Inc. 1613-1 Rabbit 1 in 500
TSC2p TSC2 phosphorylation at T1462 Cell Signaling Technology, Inc. CS 3617 Rabbit 1 in 200
VEGFR2 KDR2/VEGF Receptor 2 Cell Signaling Technology, Inc. CS 2479 Rabbit 1 in 700
XIAP X linked inhibitor of apoptosis Cell Signaling Technology, Inc. CS 2042 Rabbit 1 in 200
  1. aCompanies—Abcam, Inc. (Cambridge, MA), BD Biosciences (San Jose, CA), Cell Signaling Technology, Inc. (Danvers, MA), Dako (Carpinteria, CA), Epitomics, Inc. (Burlingame, CA), Santa Cruz Biotechnology, Inc. (Santa Cruz, CA), Upstate Biotechnology, Inc. (Millipore)