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Table 4 Time-dependent variability in total and phospho (p) protein expression with increasing time to breast tumor freezing

From: A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

Apoptosis: Cleaved caspase 7, cleaved PARP
Energy sensor pathway: AcCoAp (i.e., phospho-AcCoA), AMPKp, TSC2, TSC2p
Hormonal signaling: ERp167, PR
Phosphatidylinositol-3-kinase (PI3K) pathway: Aktp308, p110 alpha, PTEN
Src-/mitogen-activated protein kinase (MAPK) pathway: MAPKp, p38, p38p180_182, srcp527
Translation: total p70S6 Kinase, S6p235–236
Other: B catenin, COX2, E cadherin, stat3p705
  1. The expression of 21/82 total and phosphoproteins displayed significant (at p ≤ 0.05) time-dependent variability with increasing time to tumor freezing up to 24 h. These 21 proteins are subdivided by function in this table. Of all 82 assessed proteins, the 13 proteins that showed a 40% or greater percentage change from baseline with increasing time to freezing are underlined in this table