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Table 6 Reproducibility associated with biologic replicates in reverse phase protein arrays (RPPA)

From: A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers

Antibody Correlation coefficient
AcCoAp 0.642918568
Akt 0.618759766
Aktp308 0.254004137
Aktp473 0.410063812
AMPK 0.513727089
AMPKp 0.536678994
B catenin 0.730700092
BADp 0.369340325
CCNB1 0.870882305
CCND1 0.625891268
Cleaved caspase 7 0.633270435
E cadherin 0.6183121
EGFR 0.68801607
EGFRp1068 0.405450715
ER 0.841639703
ERK2 0.736704897
ERp118 0.430508819
FKHRL1p318 0.691993326
GSK3 0.678269861
GSK3p21_9 0.592290954
HER2 0.217455474
HER2p1248 0.403034203
IGFR1 0.595481674
IGFR1p 0.436972091
JNK 0.424603378
JNKp183_5 0.543731864
MAPKp 0.79987626
MEK 0.579451091
MEK1-2p 0.659646302
mTOR 0.626602561
p110alpha 0.436998926
p27 0.849943011
p38 0.716704432
p38p180_2 0.608686332
p53 0.655654172
p70S6 Kinase 0.649534728
p70S6Kp389 0.115625786
PKCalphap657 0.58393973
pmTOR 0.006433235
PR 0.758475654
PTEN 0.529437664
Rab25 0.769013148
S6p235–236 0.720622398
S6p240_4 0.866983533
Src 0.71789969
srcp416 0.210019805
srcp527 0.625513318
stat3p705 0.539502613
stat3p727 0.550006586
stat6p 0.287410482
TSC2 0.647454784
TSC2p 0.538756346
  1. Correlation coefficients for the expression of 52 proteins and phosphoproteins across two independent sections obtained from each of 49 frozen human hormone receptor-positive breast cancers are shown. Cutoff for significance—0.282 (p = 0.05), 0.46 (p = 0.001)