Figure 1From: Proteomic analysis of ERK1/2-mediated human sickle red blood cell membrane protein phosphorylationOverview of the (A) experimental design and (B) analytical strategy of the label-free quantitative phosphoproteomic workflow. RBC membrane ghosts from healthy (AA) or sickle (SS) patients were proteolytically digested and then subjected to a TiO2 based phosphopeptide enrichment. Following LC-MS/MS analysis, all data files were subjected to AMRT alignment within Rosetta Elucidator and selected ion chromatograms were generated from each phosphopeptide precursor ion to measure abundance.Back to article page