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Figure 1 | Clinical Proteomics

Figure 1

From: Proteomic analysis of ERK1/2-mediated human sickle red blood cell membrane protein phosphorylation

Figure 1

Overview of the (A) experimental design and (B) analytical strategy of the label-free quantitative phosphoproteomic workflow. RBC membrane ghosts from healthy (AA) or sickle (SS) patients were proteolytically digested and then subjected to a TiO2 based phosphopeptide enrichment. Following LC-MS/MS analysis, all data files were subjected to AMRT alignment within Rosetta Elucidator and selected ion chromatograms were generated from each phosphopeptide precursor ion to measure abundance.

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