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Figure 2 | Clinical Proteomics

Figure 2

From: Proteomic analysis of kidneys from selenoprotein M transgenic rats in response to increased bioability of selenium

Figure 2

2-DE protein patterns in kidney tissues from CMV/EGFP-hSelM Tg and non-Tg rats. Kidney lysates (1 mg) from four groups, including vehicle-treated non-Tg rats (A), Sel-treated non-Tg rats (B), vehicle-treated CMV/hSelM Tg rats (C), and Sel-treated CMV/hSelM Tg rats (D), were subjected to one-dimensional IEF using 24- cm IPG strips in a pH range from 3–10 (nonlinear). Two-dimensional SDS-PAGE was performed on 8–18 % linear gradient acrylamide gels in an EttanDalt system. Protein spots were visualized by staining with Coomassie blue G-250.

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Clinical Proteomics

ISSN: 1559-0275