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Table 1 List of differentially expressed proteins in the four experimental groups

From: Proteomic analysis of kidneys from selenoprotein M transgenic rats in response to increased bioability of selenium

Spot No.

Protein name

Gene name

Accession No.

Sequence coverage (%)

Mw(Da)/pI

Mascot

Vehicle

Selenium

Score

non-Tg

Tg*

non-Tg*

Tg*

1

Cytosol aminopeptidase (EC 3.4.11.1) (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase) (Leucine aminopeptidase 3) (Proline aminopeptidase) (EC 3.4.11.5) (Prolyl aminopeptidase)

Lap3

Q68FS4

42%

56115/6.77

25

1

2.3± 0.24

1.8± 0.31

1.3± 0.09

2

Brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1 (BAI1-associated protein 2-like protein 1)(BAIAP2L1)

Baiap2l1

Q3KR97

46%

57432/8.95

29

1

2.1± 0.17

1.7± 0.38

1.5± 0.12

3

Uncharacterized protein KIAA1143 homolog (KIAA1143 homolog)

 

Q5RKH3

52%

17442/8.51

28

1

2.4± 0.13

1.5± 0.26

1.3± 0.2

4

5’-nucleotidase precursor (EC 3.1.3.5) (Ecto-5’-nucleotidase) (5’-NT) (CD73 antigen)

Nt5e

P21588

64%

63928/6.51

48

1

1.8± 0.31

1.5± 0.22

1.4± 0.16

5

Cysteine and glycine-rich protein 2 (Cysteine-rich protein 2) (CRP2) Smooth muscle cell LIM protein) (SmLIM)

Csrp2

Q62908

64%

20926/8.95

27

1

1.9± 0.16

1.6± 0.15

1.5± 0.24

6

Platelet-derived growth factor D precursor (PDGF D) (Iris-expressed growth factor) (Spinal cord-derived growth factor B) (SCDGF-B)

Pdgfd

Q9EQT1

35%

42782/8.11

25

1

2.3± 0.35

1.6± 0.21

0.9± 0.13

7

Phosphoribosyl pyrophosphate synthetase-associated protein 2 (PRPP synthetase-associated protein 2)(PRPPS-AP2)(41 kDa phosphoribosypyrophosphate synthetase-associated protein) (PAP41)

Prpsap2

O08618

67%

40840/6.73

50

1

2.6± 0.24

1.3± 0.21

0.9± 0.08

8

Zinc finger protein 313 (ZFP313)

Znf313

Q6J2U6

39%

25647/6.38

38

1

2.7± 0.33

0.9± 0.12

0.9± 0.25

9

60 kDa heat shock protein, mitochondrial precursor (Heat shock protein 60) (HSP-60) (Hsp60) (60 kDa chaperonin) (Chaperonin 60) (CPN60) (Mitochondrial matrix protein P1) (HSP-65)

Hspd1

P63039

20%

60917/5.91

86

1

1.9± 0.15

1.1± 0.14

1.0± 0.06

10

Neural Wiskott-Aldrich syndrome protein (N-WASP)

Wasl

O08816

27%

54291/8.33

33

1

3.2± 0.34

1.2± 0.21

0.9± 0.25

11

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 (EC 1.14.11.) (Alkylated DNA repair protein alkB homolog 3)(ALKDH3)

Alkbh3

Q5XIC8

60%

33990/8.53

30

1

0.5± 0.06

0.3± 0.04

0.4± 0.03

12

Chymase precursor (EC 3.4.21.39) (Alpha-chymase) (Mast cell protease 3) (Mast cell protease III) (rMCP-III) (rMCP-3) (Mast cell protease 5) (rMCP-5)

Cma1

P50339

20%

27551/9.47

32

1

0.4± 0.03

0.9± 0.20

0.2± 0.03

13

Stanniocalcin-1 precursor (STC-1)

Stc1

P97574

43%

27490/8.47

32

1

0.3± 0.04

0.2± 0.03

0.9± 0.26

  1. *The value in the last 3 columns on the right hand side was expressed as the relative value of spot volume for the non-Tg vehicle group which was defined as 1.