Figure 2
From: Analysis of serum protein glycosylation by a differential lectin immunosorbant assay (dLISA)
Schematic representation of the dLISA approach. (A) A serum sample is split into five aliquots (A1 to A5). (B) Four of these aliquots are treated with increasing amounts of magnetic beads coupled with TIMP-1 antibody to gradually reduce the amount of endogenous TIMP-1 present. For aliquot A5, there should be sufficient beads used for complete removal of the endogenous TIMP-1. (C) After the magnetic beads treatment, the beads were separated using a magnetic separator and the supernatant from each aliquot is transferred. (D) Both TIMP-1 UEA LISA assay and TIMP-1 immunoassay are performed on each aliquot. (E) A dose–response curve is constructed using the measured TIMP-1 concentration (ng/mL) in each aliquot by the TIMP-1 immunoassay along with the corresponding fluorescence readout by the TIMP-1 UEA LISA assay.