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Figure 3 | Clinical Proteomics

Figure 3

From: Quantitative proteomic analysis of amniocytes reveals potentially dysregulated molecular networks in Down syndrome

Figure 3

A schematic representation of SRM method development for candidates and controls. Differential expression of SOD1 protein and development of SRM assay. (A) MS1 spectrum of peptide GDGPVQGIINFEQK in the equimolar mixture of digested lysates of normal (light) and SILAC-labeled Down syndrome (heavy) amniocytes revealed differential expression of SOD1 protein. MS/MS spectrum of peptide GDGPVQGIINFEQK acquired with LTQ-Orbitrap confirmed peptide identity and showed relative intensity of y- and b-fragment ions. (B) Survey SRM for peptide GDGPVQGIINFEQK in the triple quadrupole included 8 y-ion transitions, and the three most intense transitions were chosen for the final SRM assay.

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