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Figure 1 | Clinical Proteomics

Figure 1

From: Development and evaluation of an immuno-MALDI (iMALDI) assay for angiotensin I and the diagnosis of secondary hypertension

Figure 1

iMALDI workflow. Stable-isotope labelled internal standard (SIS)-Ang-I (green) is spiked into plasma and the sample is incubated with anti-Ang-I-antibody conjugated beads. After immunoprecipitation of endogenous (blue) and SIS-Ang-I the beads can be placed directly on a MALDI target. To elute the analytes and permit MALDI-MS analysis, CHCA matrix is applied. The relative abundances of endogenous to SIS-Ang-I are used for quantitation.

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