Figure 4

Immunohistochemical validation for CAMK2A on human cerebral cortex. A: Normal cortex showing labeling of soma of pyramidal neurons extending along the apical dendrite with diffuse but light synaptic pattern of labeling of the neuropil. B: Encephalitic rabies. Hippocampal pyramidal neurons from a case of encephalitic rabies reveal presence of CAMK2A as clumps in the cytoplasm appearing to localize within intracytoplasmic negri bodies (proteomics: 1.6 fold elevation). Inset shows characteristic intracytoplasmic eosinophilic Negri bodies diagnostic of rabies (arrows). C, D: Paralytic rabies. In cases of paralytic rabies, labeling with CMAK2A was seen within hippocampal pyramidal neurons, aggregating as coarse granular or clump-like fashion in addition to diffuse labeling of the cytoplasm and neuropil, corresponding to localization of rabies viral antigen (Inset, arrows). This was more intense in vaccinated cases of paralytic rabies (D) compared to non vaccinated cases (C) (proteomics: 2.08 fold elevation in non vaccinated form, 4.6 fold elevation in vaccinated rabies). [A: ImmunoperoxidasexObj.20, B: xObj.40, B, inset: H&ExObj.40B, C: Obj.x20, C, inset: Immunoperoxidase to rabies viral antigenxObj.40, D: xObj.20].