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Figure 1 | Clinical Proteomics

Figure 1

From: Protein expression profiling of nuclear membrane protein reveals potential biomarker of human hepatocellular carcinoma

Figure 1

Targeting CYB5A as potential biomarker. (A): in this representative expression profile of significantly up (ATPB, FIBB, and QCR1) and down regulated (CYB5A, ATPD and HBB) proteins in HCC compared with fibrotic liver and HepG2 cell line. Focused on IPG strips pH 3–11 NL (11 cm) and separated on 12.5% gels followed by silver staining. Gel analysis was performed by using Progenesis SameSpots v4.5 (Nonlinear Dynamic, UK). The statistical analysis using Student t-test was performed and on the basis of normalized volumes spots were selected with p-values (< 0.05). (B): Representative protein spot of CYB5A (indicated by circle), showing differential expression between HCC, fibrotic liver and HepG2 cell line. The protein spots were analyzed by using ESI-QTOF MS/MS. (C): Representative MS/MS spectrum and peptide sequence of CYB5A identified by one or more high scoring peptides were considered to be true matches or with extensive homology (p < 0.05). b and y represent b ions and y ions respectively, generated during peptide fragmentation in MS/MS. (D): Amino acid sequence of CYB5A, matched peptides were expressed in red type and underline.

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