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Figure 3 | Clinical Proteomics

Figure 3

From: Protein expression profiling of nuclear membrane protein reveals potential biomarker of human hepatocellular carcinoma

Figure 3

Antibody based identification of CYB5A as S-nitrosylated protein. (A): 2DE Spot representative differential expression level of CYB5A as S-nitrosylated protein in HCC compared with respective fibrotic liver and HepG2 cell line. The proteins were immuno-precipitated with anti SNO-Cys antibody, focused on IPG strips (pI 3-11NL; 7 cm) and separated on 12.5% gel followed by silver staining. (B): Representative spots of CYB5A immuno-precipitated with anti SNO-Cys antibody. 100 μg proteins were focused using pI 3-11NL (7 cm) IPG strips and separated on 12.5% gel followed by western blotting described in material and methods. Densitrometric analysis was performed using Progenesis SameSpots v4.5 (Nonlinear Dynamic, UK).

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