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Figure 4 | Clinical Proteomics

Figure 4

From: Protein expression profiling of nuclear membrane protein reveals potential biomarker of human hepatocellular carcinoma

Figure 4

Immunofluorescence analysis of CYB5A in HCC. The IHC image of CYB5A staining in control (A) and hepatocellular carcinoma (C &E) formalin fixed paraffin embedded (FFPE) section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer for 30min. The section was then incubated with CYB5A, 5 μg/ml, for 1hr at 40oC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 30min at 40oC. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM. (B, D & F): combination of DIC images and IHC of control and HCC respectively, work done by Adobe Photoshop CS2. (C=Portal tract; H=Normal hepatocytes; T=Malignant hepatocytes; Original magnification= 20X). IHC: Immunohistolocalization.

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