Figure 4From: Protein expression profiling of nuclear membrane protein reveals potential biomarker of human hepatocellular carcinomaImmunofluorescence analysis of CYB5A in HCC. The IHC image of CYB5A staining in control (A) and hepatocellular carcinoma (C &E) formalin fixed paraffin embedded (FFPE) section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer for 30min. The section was then incubated with CYB5A, 5 μg/ml, for 1hr at 40oC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 30min at 40oC. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 μM. (B, D & F): combination of DIC images and IHC of control and HCC respectively, work done by Adobe Photoshop CS2. (C=Portal tract; H=Normal hepatocytes; T=Malignant hepatocytes; Original magnification= 20X). IHC: Immunohistolocalization.Back to article page