Figure 1
From: Addressing the needs of traumatic brain injury with clinical proteomics
Candidate biomarker discovery and verification workflow. Bottom-up proteomics strategies, such as shotgun proteomics (multidimensional LC-MS/MS) and 2D-PAGE/MS, can be applied to identify putative candidate markers (left). Candidate protein markers can be subsequently verified and confirmed by targeted proteomics using standard ELISA methods or multiple reaction monitoring (MRM)-MS (right). MRM-MS offers the advantages of an antibody-independent platform with capabilities for multiplexing.