Figure 3

TTR isoform data extraction. Chromatographic elution profiles from the 3 hour (A) and the 6 hour (B) samples (from Figure 2). The TTR isoforms elute with substantial overlap (A1 and B1), from 28 to 34 min. In the panels below (A2 and B2) extracted chromatograms are presented for all four TTR isoforms and differential peak elution times can be observed. For peak extractions the theoretical isoform masses were calculated and the m/z windows were manually adjusted according to the precision of the external calibration. The calibration was minor, routinely 0–3 ppm. Spectra across the four elution peaks were combined (A3 and B3). These raw spectra were used for deconvolution and subsequent semi-quantitative calculations as described in Materials and Methods.